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目的 :对比地尔硫 (DTZ)及过氧岐化酶 (SOD)对心肌缺血再灌注损害的保护作用 ,以及明确联合应用 SOD+DTZ的疗效是否比单一药物更好。方法 :将 37只小型猪造成在体心肌缺血再灌注模型 ,分为 4组。 组 10只于再灌注前5分钟经静脉注射生理盐水作为对照组 , 组 9只再灌注前 5分钟静脉注射 SOD, 组 8只于再灌注前 5分钟静脉注射 DTZ, 组 9只于再灌注前 5分钟静脉注射 DTZ+SOD。结果 :(1)心梗范围 :用药各组与对照组比较 ,均有显著性缩小 ,DTZ+SOD组与 SOD组及 DTZ组比较 ,时性均有显著缩小 ;(2 )心肌钙离子含量 :SOD组与对照组比较有降低 ,但无显著性差异 ,而 DTZ组及 SOD+DTZ组与对照组比较均有显著的降低 ;(3) DTZ组及 SOD+DTZ组均可降低心率 ,减少再灌注性心律失常 ;(4)用药组均有降低灌注后 CK- MB的作用 ,但 SOD组的降低效果不明显 ,与对照组比较 ,无显著差异 ,而 DTZ组与对照组比较时性有显著性降低 ;SOD+DTZ组与 SOD组及 DTZ组比较均有显著性降低 ;(5 )电镜心肌超微结构观察显示 :心肌细胞损伤程度对照组比用药组严重 ,SOD组比 DTZ组及 SOD+DTZ组严重 ,DTZ组比 SOD+DTZ组严重。结论 :SOD、DTZ对缺血再灌注心肌有明确的保护作用 ,但 DTZ效果优于 SOD,合用 SOD+DTZ较应用其中单一药物效果更好。
Objective: To compare the protective effects of diltiazem (DTZ) and superoxide dismutase (SOD) on myocardial ischemia-reperfusion injury and to determine whether the combined effect of SOD + DTZ is better than that of single drug. Methods: 37 miniature pigs were subjected to myocardial ischemia-reperfusion model and divided into 4 groups. Group 10 received intravenous injection of normal saline 5 minutes before reperfusion as control group, 9 rats received intravenous injection of SOD 5 minutes before reperfusion, 8 rats received intravenous DTZ 5 minutes before reperfusion, and 9 rats received intravenous injection of 5 minutes intravenous DTZ + SOD. Results: (1) The range of myocardial infarction: Compared with the control group, all the groups in the treatment group showed significant reduction. The DTZ + SOD group showed a significant decrease compared with the SOD and DTZ groups. (2) The content of calcium in the myocardium: Compared with the control group, the SOD and DTZ groups decreased significantly, but there was no significant difference between the DTZ group and SOD + DTZ group. (3) DTZ group and SOD + DTZ group decreased heart rate and decreased Perfusion arrhythmia; (4) The treatment group were reduced the role of CK-MB after perfusion, but the effect of SOD was not significantly reduced, compared with the control group, no significant difference, while DTZ group compared with the control group was significant (5) The myocardial ultrastructure observation showed that the degree of cardiomyocyte injury in the control group was more serious than that in the drug-treated group, SOD group was more than the DTZ group and SOD + The DTZ group was severe, and the DTZ group was more severe than the SOD + DTZ group. CONCLUSIONS: SOD and DTZ have a definite protective effect on myocardial ischemia-reperfusion injury, but the effect of DTZ is better than that of SOD. Combined with SOD + DTZ is better than single drug.