目的:分析Plk1基因在肝细胞癌(hepatocellular carcinoma,HCC)中的表达,及其与预后的关系.方法:用半定量RT-PCR及Western blot方法检测我院2003-01/2008-05原发性肝癌患者213例的Plk1基因蛋白表达,同时应用Kaplan-Meier法及多变量Cox比例险模型,分析Plk1基因的表达及临床病理因素与预后的关系.结果:Plk1基因在肝癌组织中阳性表达率为83.6%.Plk1阳性组的1、3、5年的生存率明显低于阴性组(P=0.004).原发性肝癌的预后与Plk1阳性表达,Edmondson分级,肉眼癌栓,显微癌栓及肿瘤数目相关;与HBsAg、肝硬化、AFP、假包膜、肿瘤大小无关;多因素Cox回归分析显示Edmondson分级、Plk1基因蛋白表达、肉眼癌栓及肿瘤数目4项指标反映肝癌预后情况,危险度分别为1.717、1.938、1.537及2.355.结论:Plk1基因的检测有助于有高危肝癌患者的选择,为Plk1基因靶向治疗肝癌提供了有力依据. OBJECTIVE: To analyze the expression of Plk1 in hepatocellular carcinoma (HCC) and its relationship with prognosis.Methods: We detected the expression of Plk1 in primary hepatocellular carcinoma (HCC) from January 2003 to May 2008 by semi-quantitative RT-PCR and Western blot Plk1 gene protein was detected in 213 cases of HCC, Kaplan-Meier method and multivariate Cox proportional hazard model were used to analyze the relationship between Plk1 gene expression and clinical pathological factors and prognosis.Results: The positive rate of Plk1 in HCC (P = 0.004) .The prognosis of primary hepatocellular carcinoma was positively correlated with the expression of Plk1, Edmondson grade, macroscopic embolism and micro-tumor thrombus And the number of tumor. There was no relationship with HBsAg, cirrhosis, AFP, pseudocapsule and tumor size. Multivariate Cox regression analysis showed that Edmondson classification, Plk1 protein expression, macroscopic embolism and tumor number reflected the prognosis, risk Degrees were 1.717,1.938,1.537 and 2.355 respectively.Conclusion: The detection of Plk1 gene is helpful for the selection of patients with high-risk liver cancer and provides a strong basis for the targeted therapy of liver cancer with Plk1 gene.