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目的建立野百合碱诱导的肺动脉高压的大鼠模型,观察丹参酮ⅡA对大鼠血清中的白细胞介素6(interleukin 6,IL-6)和IL-8的表达的影响,探讨丹参酮ⅡA对防治肺动脉高压的意义。方法 SD大鼠分为野百合碱组、丹参酮ⅡA治疗组及对照组,野百合碱组给予大鼠肌肉注射野百合碱制成肺动脉高压大鼠模型,丹参酮ⅡA治疗组在注射野百合碱的基础上给予注射丹参酮ⅡA,对照组不作处理。分别测定3组右心室收缩压(right ventricular systolic pressure,RVSP)、平均肺动脉压力(mean pulmonary arterial hypertension,mPAP)、右心室肥厚指数(right ventricle hypertrophy index,RVHI)。用酶联免疫法(enzyme linked immunosorbent assays,ELISA)检测血清中的IL-6和IL-8的含量。结果成功建立了野百合碱诱导的肺动脉高压大鼠模型,使用丹参酮ⅡA可以减低野百合碱引起的肺动脉压力的升高及IL-6和IL-8的表达。结论 IL-6及IL-8参与了野百合碱诱导的肺动脉高压,丹参酮ⅡA可以降低野百合碱诱导的肺动脉高压,可能与减轻肺动脉高压的炎症反应有关。
Objective To establish a rat model of monocrotaline-induced pulmonary hypertension and observe the effect of tanshinone ⅡA on the expression of interleukin 6 (IL-6) and interleukin-6 (IL-8) in rat serum and to explore the effect of tanshinone ⅡA on prevention and treatment of pulmonary artery The meaning of high pressure. Methods SD rats were divided into monocrotaline group, tanshinone Ⅱ A treatment group and control group, monocrotaline group was given intramuscular injection of monocrotaline into rat model of pulmonary hypertension, tanshinone Ⅱ A treatment group in the injection of monocrotaline basis On the injection of tanshinone Ⅱ A, the control group without treatment. Three groups of right ventricular systolic pressure (RVSP), mean pulmonary arterial hypertension (mPAP) and right ventricular hypertrophy index (RVHI) were measured. Serum levels of IL-6 and IL-8 were detected by enzyme linked immunosorbent assays (ELISA). Results The rat model of pulmonary hypertension induced by monocrotaline was successfully established. Tanshinone ⅡA can reduce the increase of pulmonary arterial pressure and IL-6 and IL-8 induced by monocrotaline. Conclusion IL-6 and IL-8 are involved in monocrotaline-induced pulmonary hypertension. Tanshinone IIA can reduce monocrotaline-induced pulmonary hypertension, which may be related to the reduction of inflammatory reaction of pulmonary hypertension.