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目的 探讨宫颈癌及癌前病变与肿瘤组织中P63 和CD44v6 表达调控的关系及其临床意义, 为宫颈癌的早期诊断和临床治疗提供依据.方法 收集宫颈上皮组织标本74 例, 其中官颈鳞癌(cervical squamous cell carcinoma, CSCC) 患者36 例, 宫颈上皮内瘤变(cervical intraepithelial neoplasia, CIN) Ⅱ ～Ⅲ患者13 例及正常对照25 例, 提取组织总RNA, 利用荧光定量RT - PCR 方法鉴定P63 和CD44v6 基因的转录表达水平.结果 P63 和CD44v6 基因在宫颈组织恶性转化过程中, 随着宫颈病变程度的加重, 两种基因的表达水平逐渐升高, 与病变程度呈正相关(P ＜ 0. 05); 各组间两两比较发现, CSCC 与NC 或CIN 比较, 上述两种基因的表达差异有统计学意义(P ＜ 0. 05); 而CIN 与NC 比较, P63 和CD44v6 表达水平差异无统计学意义(P ＞ 0. 05); 对P63和CD44v6 基因表达水平变化的关系进行Pearson 相关性分析, 发现该两种基因呈中度相关(r = 0. 320, P = 0. 018).单一基因表达水平检测对宫颈癌诊断的特异性和敏感度分析, 两种基因的曲线下面积均＞ 0. 7, 说明上述基因检测的诊断准确度较高, 对宫颈癌有一定的预警价值, 推测宫颈癌发生与P63 和CD44v6 基因表达水平升高存在密切关系.结论 P63 和CD44v6 等干细胞标志物的表达调控可能成为宫颈癌的候选生物学标志物, 对于宫颈癌早期预警和临床治疗有重要意义, 联合检测可以为宫颈癌判断预后提供重要依据.“,”Objective To study the association of cervical cancer and precancerous lesions in Uyghur women with the expression of P63 and CD44 genes, and to probe the mechanism of cervical carcinogenesis and early prognostic markers. Methods We collected 74 cases of fresh tissue specimens from Uyghur women with cervical lesions, including 36 cases of cervical squamous cell carcinoma (CSCC), 13 cases of cervical intraepithelial neoplasia(CIN) and 25 cases of cervicitis or normal cervix (NC). Total RNA extracted from the tissue samples. The mRNA level of cervical cancer and precancerous lesions was analyzed by quantitative RT-PCR. Results The results showed that, the expression of all genes above was up-regulated with the development of cervical lesions. Significant differences was found in all genes above between CSCC and CINⅡ -Ⅲor cervicitis (P ＜ 0. 05). Compared with CIN and NC, no significant differences were found in P63 and CD44 gene expression(P ＞ 0. 05). The analysis of changes in the expression of P63 and CD44 gene during cervical cancer development by Pearson correlation showed that P63 gene expression was moderately correlated with CD44 gene(r = 0. 320, P = 0. 018). The analysis of specificity and sensitivity in the detection above genes expression showed that the detection of P63 and CD44 had a high accuracy based on AUC which was bigger than 0. 70, suggested that the detection of above genes have an impact in early prognosis of cervical cancer. We can infer P63 and CD44 expression differences could become cervical cancer early warning markers. Conclusion The occurrence and development of cervical cancer is closely related with invasion and metastasis of P63and CD44v6 genes. Above genes may be a candidate biomarker for cervical cancer. The joint detection of P63 and CD44 genes could serve as early diagnosis marker for cervical cancer or precancerous lesion;