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目的:建立高效液相色谱法同时测定速克感冒胶囊中绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素。方法:采用Venusil XBP C_(18)色谱柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.5%磷酸溶液(B)为流动相,梯度洗脱,流速1.0 mL·min~(-1),检测波长327 nm(绿原酸)、260 nm(氯苯那敏)、276 nm(乙酰水杨酸)、334 nm(蒙花苷)和266 nm(次野鸢尾黄素)。结果:5种待测成分分离度良好,阴性无干扰;绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素进样量分别在0.078~0.388μg(r=0.999 9)、0.196~0.978μg(r=0.999 7)、4.066~20.332μg(r=0.999 9)、0.165~0.825μg(r=0.999 9)和0.048~0.238μg(r=0.999 0)范围内线性关系良好;平均回收率分别为96.8%、101.2%、96.8%、100.0%和101.3%,RSD分别为1.8%、2.4%、1.1%、1.9%和3.9%。3批样品中绿原酸、马来酸氯苯那敏、乙酰水杨酸、蒙花苷和次野鸢尾黄素的平均含量分别为0.88~1.00、2.05~2.20、110.02~116.00、2.18~2.45和0.06~0.10 mg·粒~(-1)。结论:该方法可作为评价和监控速克感冒胶囊质量的方法。
OBJECTIVE: To establish a HPLC method for the simultaneous determination of chlorogenic acid, chlorpheniramine maleate, acetylsalicylic acid, malathion and immature tectorigenin in Suck’s cold capsule. Methods: The gradient elution was carried out on a Venusil XBP C 18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase of acetonitrile (A) - 0.5% phosphoric acid (B) at a flow rate of 1.0 mL · min ~ 1). The detection wavelength was 327 nm (chlorogenic acid), 260 nm (chlorpheniramine), 276 nm (acetylsalicylic acid), 334 nm (monoglyceride) and 266 nm (myrosin). Results: The five analytes were well separated and negative without interference. The injection volumes of chlorogenic acid, chlorpheniramine maleate, acetylsalicylic acid, mandarin glycosides and nitrendipine were 0.078-0.388 μg = 0.999 9), 0.196-0.978 μg (r = 0.999 7), 4.066-20.332 μg (r = 0.999 9), 0.165-0.825 μg (r = 0.999 9), and 0.048-0.238 μg The average recovery was 96.8%, 101.2%, 96.8%, 100.0% and 101.3%, respectively. The RSDs were 1.8%, 2.4%, 1.1%, 1.9% and 3.9%, respectively. The average contents of chlorogenic acid, chlorpheniramine maleate, acetylsalicylic acid, mandarin glycosides and nitrendiphos in the three batches were 0.88-1.00, 2.05-2.20, 110.02-116.00, 2.18-2.45 And 0.06 ~ 0.10 mg · grain ~ (-1). Conclusion: This method can be used as a method to evaluate and monitor the quality of Sox capsules.