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目的用脉冲场凝胶电泳(PFGE)分型技术,研究四川省2007年鼠伤寒沙门菌分子流行病学,查找沙门菌污染源,为预测疫情和制定防治措施提供依据。方法选择XbaI酶对12株鼠伤寒沙门菌全基因组DNA进行酶切,用PFGE对菌株进行分子分型,Bionumerisc统计软件聚类分析。结果12株鼠伤寒沙门菌用XbaI限制性酶切后,分成5个PFGE图谱类型,其中1个PFGE图谱类型有7株菌株,其指纹图谱的相似性达到100%,该型别占分析菌株的58.33%。其余4个PFGE图谱类型分别有2株菌和1株菌。含7株菌的PFGE图谱型中,源于成都市的菌株4株,攀枝花、自贡和内江市的菌株各1株。结论PFGE分子分型与流行病学资料紧密结合可增强对鼠伤寒沙门菌食源性疾病的溯源和预警。
Objective To investigate the molecular epidemiology of Salmonella typhimurium in Sichuan Province by using pulsed-field gel electrophoresis (PFGE) typing and to find the sources of Salmonella contamination, and to provide a basis for predicting the epidemic situation and making prevention and control measures. Methods Whole genome DNA of 12 Salmonella typhimurium strains was digested by XbaI enzyme, and the strains were genotyped by PFGE and clustered by Bionumerisc statistical software. Results 12 strains of Salmonella typhimurium were digested with restriction enzyme XbaI and divided into 5 PFGE patterns. Among them, 7 strains of one PFGE pattern shared 100% similarity in fingerprinting, 58.33%. The other four PFGE patterns were 2 strains and 1 strain respectively. In the PFGE pattern with 7 strains, 4 strains originated from Chengdu, 1 strain from Panzhihua, Zigong and Neijiang. Conclusion The close association of PFGE molecular typing and epidemiological data can enhance the traceability and early warning of foodborne illness of Salmonella typhimurium.