论文部分内容阅读
目的:探讨选择性环氧合酶-2(cyclooxygenase-2,COX-2)抑制剂NS-398对多药耐药细胞株K562/ADM细胞P-糖蛋白(P-glycoprotein,P-gp)表达的影响。方法:K562/ADM细胞经浓度分别为10μmol/L、20μmol/L、40μmol/L、80μmol/L、160μmol/L的NS-398处理,24h、48h、72h后用RT-PCR法检测MDR1mRNA的表达、用流式细胞仪检测mdr1蛋白表达。结果:随着NS-398浓度的升高以及作用时间延长,MDR1mRNA、p-gp表达降低,不同浓度的药物与测量时间之间存在着交互作用(P<0.05)。结论:选择性环氧合酶-2抑制剂NS-398可抑制K562/ADM细胞的MDR1/P-gp表达。
Objective: To investigate the expression of P-glycoprotein (P-glycoprotein, P-glycoprotein, P-gp) in multidrug-resistant cell line K562 / ADM with selective cyclooxygenase-2 inhibitor NS- Impact. Methods: The expression of MDR1 mRNA was detected by RT-PCR in K562 / ADM cells after treatment with NS-398 at concentration of 10μmol / L, 20μmol / L, 40μmol / L, 80μmol / L and 160μmol / The expression of mdr1 protein was detected by flow cytometry. Results: The expression of MDR1 mRNA and p-gp decreased with the increase of NS-398 concentration and the prolongation of action time. There was interaction between different concentrations of drug and measurement time (P <0.05). CONCLUSIONS: Selective cyclooxygenase-2 inhibitor NS-398 inhibits MDR1 / P-gp expression in K562 / ADM cells.