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用激光共聚焦技术在急性分离的大鼠背根神经节（DRG）神经元上观察了施加高糖溶液后罗丹明-123（Rhodamine－123）荧光强度的变化，从而推测高血糖对线粒体膜电位的作用及能量代谢的影响。对40个小DRG细胞的观察表明，罗丹明-123荧光强度在细胞的周边最高，而细胞核区的荧光强度最低。当在DRG细胞的培养液（人工脑脊液，ACSF）中滴加30mmol／L的D-葡萄糖溶液后，大部分细胞（8／9）荧光强度迅速降低，在100～400s内，平均降低21．2％±5．4％～22．8％±4．7％。而同体积的ACSF对荧光强度无明显影响（n＝7）。滴加葡萄糖后的罗丹明-123荧光强度变化曲线同滴加ACSF后的曲线相比有显著差异（p＜0．05）。说明急性高血糖可引起初级传入神经元的能且代谢降低。
The change of fluorescence intensity of rhodamine-123 after high glucose solution was administered to acutely isolated rat DRG neurons by confocal laser scanning microscopy was used to evaluate the effect of hyperglycemia on mitochondrial membrane potential The role of energy metabolism and the impact. The observation of 40 small DRG cells showed that the fluorescence intensity of rhodamine-123 was the highest in the periphery of the cell, while the fluorescence intensity in the nucleus was the lowest. When 30 mmol / L D-glucose solution was added to DRG cell culture medium (artificial cerebrospinal fluid, ACSF), the fluorescence intensity of most cells (8/9) decreased rapidly and decreased by 21.2 on average within 100-400 s % ± 5.4% ~ 22.8% ± 4.7%. The same volume of ACSF had no significant effect on the fluorescence intensity (n = 7). Fluorescence intensity of rhodamine-123 after dripping glucose was significantly different from that of dripping ACSF (p <0.05). Description of acute hyperglycemia can lead to primary afferent neurons and metabolism decreased.