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目的探讨肾上腺髓质素与脑缺血再灌注损伤的关系。方法采用栓线法制成大鼠大脑中动脉缺血再灌注模型, 阻断血流 2 h 后进行再灌注。应用免疫组织化学染色法检测不同时间段大鼠局灶性脑缺血再灌注后肾上腺髓质素的表达情况, 并进行动态观察。结果正常大鼠脑内即有肾上腺髓质表达, 假手术后肾上腺髓质素表达略有增加, 但与正常对照组相比无明显差异, P >0.05; 大鼠脑缺血再灌注后肾上腺髓质素免疫阳性细胞增多, 与正常对照组及假手术组相比差异显著, 均 P <0.05; 大鼠脑缺血再灌注后缺血侧既缺血对侧肾上腺髓质素免疫阳性细胞均增多, 但以缺血侧区域增多最为明显, P <0.05。动态观察发现, 脑缺血再灌注 2 h 后, 肾上腺髓质素免疫阳性细胞即增多, 缺血再灌注 6 h 达高峰, 至 1 周左右仍明显增多。结论脑缺血再灌注后肾上腺髓质表达增强。
Objective To investigate the relationship between adrenomedullin and cerebral ischemia-reperfusion injury. Methods The model of middle cerebral artery occlusion (MCAO) was established by the method of plug line, and the blood flow was blocked for 2 hours before reperfusion. Immunohistochemical staining was used to detect the expression of adrenomedullin after focal cerebral ischemia-reperfusion in different time periods and the dynamic observation. Results The expression of adrenomedullin was increased in the normal rat brain and the expression of adrenomedullin was slightly increased after sham operation, but there was no significant difference compared with the normal control group (P> 0.05). After adrenal medulla Compared with the normal control group and the sham operation group, there were significant differences (P <0.05). Immuno-positive cells in the ischemic and contralateral sides of the ischemic and contralateral adrenal medulla were all increased , But the most obvious increase in the ischemic area, P <0.05. Dynamic observation found that 2 h after cerebral ischemia-reperfusion, adrenal medulla immunoreactive cells that increased 6 h after ischemia-reperfusion peaked to 1 week still significantly increased. Conclusion The expression of adrenal medulla increased after cerebral ischemia-reperfusion.