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对芸薹属芸薹种不同亚种间大白菜福田50双单倍体纯系FT50和小白菜双单倍体纯系HG01杂交的F1植株进行小孢子培养,在培养基中加入不同浓度的6-BA和NAA,研究其对胚状体诱导和植株再生的影响。结果表明:最适的小孢子诱导培养基为NLN-13+0.05mg.L-16-BA+0.05mg.L-1NAA,诱导率可达14.13胚.蕾-1。最适的生根培养基为MS+0.10mg.L-1NAA+3%蔗糖+0.55%琼脂,生根率为100.00%,再生植株驯化后移栽到到花盆中,在日光温室培育,采取形态学和流式细胞仪结合方法鉴定再生植株倍性,自然加倍率为56.67%。人工蕾期自交得到一个具有242个双单倍体的作图群体。
The Brassica brassicae different subspecies Chinese cabbage Fukuda 50 double haploid pure line FT50 and Chinese cabbage double haploid HG01 hybrid F1 plants were microspore culture in the medium by adding different concentrations of 6 -BA and NAA to study their effects on embryoid body induction and plant regeneration. The results showed that the optimal medium for the induction of microspore was NLN-13 + 0.05mg.L-16-BA + 0.05mg.L-1NAA with an induction rate of 14.13 embryos. The best rooting medium was MS + 0.10mg.L-1NAA + 3% sucrose + 0.55% agar with the rooting rate of 100.00%. The regenerated plants were transplanted into flower pots after domestication and cultivated in sunlight greenhouse to take morphological And flow cytometry method to identify the ploidy of regenerated plants, natural magnification was 56.67%. An artificial population of 242 doubly haploid individuals was obtained from artificial bud stage.