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自Ermengen(1896)发现肉毒毒素引起肉毒中毒以来,普遍采用小鼠毒力测定和中和试验检测肉毒毒素。该法需耗费较多动物和时间,人们便致力于寻找比较快速、敏感和特异的检测方法,如琼脂扩散法、电免疫扩散、放射免疫测定、间接血凝和酶联免疫吸附试验(ELISA)等血清学方法。其中以间接血凝最为敏感和快速,可在60分钟内检出约1个小鼠LD_(50)/ml A型毒素,但影响因素多,结果不稳定。琼脂扩散不能定量。放射免疫测定的敏感高又能定量,但半衰期短,操作和设
Since Ermengen (1896) discovered that botulinum toxin causes botulism, botulinum toxin has been commonly tested in mouse virulence and neutralization assays. This method requires more animals and time, and people are devoted to find out more rapid, sensitive and specific detection methods such as agar diffusion method, electro-immunodiffusion, radioimmunoassay, indirect hemagglutination and enzyme-linked immunosorbent assay (ELISA) Serological methods. One of the most sensitive and rapid indirect hemagglutination, can be detected within 60 minutes about 1 mouse LD_ (50) / ml type A toxin, but many factors, the results are unstable. Agar diffusion can not be quantified. Radioimmunoassay sensitive and high quantitative, but short half-life, operation and design