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目的探讨COX-2的表达与星形细胞肿瘤发生、发展的关系。方法应用免疫组化技术和RT-PCR技术检测各级别星形细胞肿瘤组织及非肿瘤组织中COX-2蛋白和mRNA的表达水平。结果COX-2蛋白在各组中的表达强度(IODA值)分别为:非肿瘤组织(2.19±0.88),肿瘤组织(6.38±3.68);COX-2mRNA在各组中的表达阳性率分别为:非肿瘤组织(33.3%),肿瘤组织(83.3%);COX-2mRNA的相对含量分别为:非肿瘤组织(43.49±19.73),肿瘤组织(119.67±46.56);COX-2在肿瘤组织中的表达显著高于其在非肿瘤组织中的表达(P<0.05)。结论COX-2的过表达与星形细胞肿瘤的发生、发展关系密切,是引起肿瘤细胞恶性表型的相关基因之一;毛细胞型星形细胞瘤与弥漫性浸润性星形细胞肿瘤的遗传基础不同,是一种在生物学和形态学上所发生的基因改变不同于其它胶质瘤的星形细胞肿瘤;COX-2除参与肿瘤形成、炎症的发生等病理过程外,在人体器官发育及正常生理功能的维持过程中也起到比预期更为复杂的作用。
Objective To investigate the relationship between the expression of COX-2 and the occurrence and development of astrocytoma. Methods Immunohistochemistry and RT-PCR were used to detect the expression of COX-2 protein and mRNA in all kinds of astrocytomas. Results The expression of COX-2 protein in each group was 2.19 ± 0.88 and 6.38 ± 3.68, respectively. The positive expression rates of COX-2 mRNA in each group were: The relative content of COX-2 mRNA in non-tumor tissue (33.3%) and tumor tissue (83.3%) were as follows: non-tumor tissue (43.49 ± 19.73), tumor tissue (119.67 ± 46.56), COX-2 expression in tumor tissue Was significantly higher than that in non-tumor tissue (P <0.05). Conclusions Overexpression of COX-2 is closely related to the occurrence and development of astrocytic tumors, which is one of the genes involved in the malignant phenotype of tumor cells. The inheritance of COX-2 is associated with diffuse invasive astrocytoma Basis is different, is a biological and morphological changes occurred in different genesis of gliomas and other astrocytic tumors; COX-2 in addition to participation in tumor formation, inflammation and other pathological processes, the development of human organs And normal physiological function of the maintenance process also played a more complex than expected.