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目的:采用液相色谱-质谱联用技术鉴定大鼠给药山柰酚单体后血浆中代谢产物。方法:取给药后大鼠的血浆样品经酶水解、酸水解后,进行色谱分离,采用液相色谱-质谱联用仪检测,大气压电喷雾离子源(EPI)负离子条件下进行扫描,鉴定大鼠给药山柰酚(30 mg.kg-1)单体后血浆中代谢产物的结构。结果:通过与山柰酚对照品谱图比较,结合质谱仪扫描结果分析,在大鼠给药山柰酚单体后血浆中鉴定出山柰酚(m/z286.0)代谢产物结构。结论:采用酶水解、酸水解的方法可快速、有效地鉴定出大鼠血浆中山柰酚代谢产物,为进行含山柰酚中药及其复方的药物代谢动力学研究奠定科学基础。
OBJECTIVE: To identify the metabolites in rat plasma by using liquid chromatography-mass spectrometry (LC-MS / MS) technique. Methods: The plasma samples from rats were enzymatically hydrolyzed, acid hydrolyzed and chromatographed. The samples were analyzed by liquid chromatography-mass spectrometry (MS-ESI-MS / MS) and ionized with atmospheric pressure electrospray ionization (EPI) The structure of metabolites in plasma after administration of monophenol (30 mg.kg-1) to mice. Results: The metabolite structure of kaempferol (m / z286.0) was identified in the plasma of rats after administration of kaempferol, compared with that of kaempferol control and the mass spectrometry scan results. CONCLUSION: The metabolites of kaempferol in rat plasma can be rapidly and effectively identified by enzymatic hydrolysis and acid hydrolysis, which lays a scientific foundation for the pharmacokinetic study of kaempferol-containing traditional Chinese medicine and its compound.