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目的:探讨氢饱和生理盐水(HRS)对大鼠重症急性胰腺炎(SAP)肺损伤的影响及其与NOX2/ROS/NF-κB通路的关系。方法:72只雄性Wistar大鼠随机分为假手术组、SAP模型组(SAP组)、SAP模型+HRS治疗组(HRS组);SAP模型以胆胰管逆行注射5%牛磺胆酸钠制备;术后5min,HRS组经尾静脉注射HRS(6mL/kg),假手术组与SAP组以等体积生理盐水代替;术后3、12、24h分批剖杀大鼠,胰腺和肺组织行病理学检查,以及血清胰酶水平、肺组织丙二醛(MDA)与超氧化物歧化酶(SOD)水平、肺组织NOX2、NF-κB及TNF-α表达水平检测。结果:与假手术组比较,SAP组和HRS组大鼠各时间点均出现明显的SAP与肺损伤表现,表现为胰腺、肺组织病理学评分增加,血清胰酶水平升高,肺组织MDA含量升高、SOD活性降低(均P<0.05),但除胰酶水平外(均P>0.05),HRS组各损伤指标均明显低于SAP组(均P<0.05);术后12h免疫组化结果显示,SAP组和HRS组大鼠肺组织中NOX2、NF-κB和TNF-α的表达均较假手术组升高(P<0.05),HRS组上述指标均较SAP组明显低于SAP组降低(均P<0.05)。结论:HRS有减轻SAP大鼠肺损伤的作用,机制可能与抑制NOX2/ROS/NF-κB通路活性有关。
Objective: To investigate the effect of hydrogen saturated saline (HRS) on lung injury in rats with severe acute pancreatitis (SAP) and its relationship with NOX2 / ROS / NF-κB pathway. Methods: Seventy-two male Wistar rats were randomly divided into sham-operation group, SAP model group (SAP group) and SAP model group (HRS group). SAP model was prepared by retrograde cholangiopancreatography with 5% sodium taurocholate HRS (6 mL / kg) was injected into caudal vein at 5 min after operation, and the rats in sham operation group and SAP group were replaced by equal volume of normal saline. Rats were sacrificed at 3, 12 and 24 h after operation, and the pancreas and lung tissues Pathological examination, serum trypsin level, malondialdehyde (MDA) and superoxide dismutase (SOD) levels, lung tissue NOX2, NF-κB and TNF-α levels were detected. Results: Compared with the sham-operation group, the SAP and the lung injury group showed obvious SAP and lung injury at each time point. The pathological score of pancreas and lung increased, serum trypsin level increased, MDA content in lung tissue (All P <0.05). However, the indexes of injury in HRS group were significantly lower than those in SAP group (all P <0.05) The results showed that the expression of NOX2, NF-κB and TNF-α in lung tissue of SAP group and HRS group were significantly higher than those of sham operation group (P <0.05). The indexes of HRS group were significantly lower than those of SAP group Decreased (all P <0.05). Conclusion: HRS can attenuate the lung injury in SAP rats. The mechanism may be related to the inhibition of NOX2 / ROS / NF-κB pathway activity.