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对7只8周雄大鼠(其中5只采用冰冻切片、2只石蜡切片)的鼻咽部Waldeyer’s环淋巴样组织(WRE)以及淋巴结、集合淋巴结、脾等其他淋巴组织进行了免疫组化研究。其目的是研究WRE淋巴组织的各种淋巴细胞及非淋巴细胞的分区和分布情况。组织用异戍烷速冻,-70℃保存作冰冻切片或用SF液「6％W/V,HgCl_2,10％V/V(37％)福尔马林,5％冰醋酸蒸馏水」,该固定液的优点是固定后作石蜡切片,可不必用胰酶事先消化即可在石蜡切片中检测胞浆中免疫
Immunohistochemical studies were performed on Waldeyer’s ring-like lymphoid tissue (WRE) from 7 8-week-old male rats (5 frozen sections and 2 paraffin sections) and other lymphoid tissues such as lymph nodes, lymph nodes and spleen. Its purpose is to study the zoning and distribution of various lymphocytes and non-lymphocytes in the WRE lymphoid tissue. The tissue was snap frozen with isoparaffin, stored frozen at -70 ° C, or treated with SF solution “6% w / v, HgCl 2, 10% v / v formalin, 5% glacial acetic acid distilled water” Liquid has the advantage of paraffin fixed after slicing can be pre-digested with pancreatin can be detected in paraffin sections of cytoplasm immunity