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目的探讨蛋白激酶CK2α在子宫内膜腺癌中的表达及其临床意义。方法 36例人子宫内膜腺癌和癌旁正常组织标本,其患者年龄44~67岁,中位年龄57.5岁。子宫内膜腺癌按2000年国际妇产科联盟(FIGO)分期标准进行临床分期:Ⅰ期19例(其中肌层浸润≤1/2为11例,>1/2为8例),Ⅱ期9例,Ⅲ、Ⅳ期8例。组织学分级按2000年FIGO子宫内膜腺癌3级分类法:Ⅰ级11例,Ⅱ级15例,Ⅲ级10例。采用逆转录聚合酶链反应及免疫组织化学方法检测癌组织标本和其癌旁正常组织中CK2αmRNA及蛋白表达水平,分析CK2α蛋白表达与子宫内膜腺癌临床病理特征之间关系。结果 CK2αmRNA在子宫内膜腺癌组织中表达量明显高于癌旁正常组织,而CK2α蛋白在子宫内膜腺癌组织中表达阳性率亦明显高于癌旁正常组织,但CK2α蛋白表达与临床分期、组织学分级及肌层浸润程度无关。结论 CK2α表达或活性增加与子宫内膜腺癌发生密切相关,CK2α可能是治疗子宫内膜腺癌的潜在分子靶点。
Objective To investigate the expression of protein kinase CK2α in endometrial adenocarcinoma and its clinical significance. Methods Thirty - six cases of endometrial adenocarcinoma and adjacent normal tissue samples from 44 to 67 years old, with a median age of 57.5 years. Endometrial adenocarcinoma according to the 2000 International Federation of Gynecology and Obstetrics (FIGO) staging staging: 19 cases of stage Ⅰ (myometrial invasion ≤ 1/2 in 11 cases,> 1/2 of 8 cases), stage Ⅱ 9 cases, Ⅲ, Ⅳ 8 cases. Histological grade According to the 2000 FIGO endometrial adenocarcinoma three-level classification: Ⅰ grade in 11 cases, Ⅱ grade in 15 cases, Ⅲ grade in 10 cases. The expression of CK2αmRNA and protein in cancer tissues and adjacent normal tissues were detected by reverse transcription polymerase chain reaction and immunohistochemistry. The relationship between CK2αprotein expression and clinicopathological features of endometrial adenocarcinoma was analyzed. Results The expression level of CK2α mRNA in endometrial adenocarcinoma was significantly higher than that in adjacent normal tissues. The positive rate of CK2α protein in endometrial adenocarcinoma was also significantly higher than that in adjacent normal tissues. However, CK2α protein expression and clinical stage , Histological grade and the degree of myometrial infiltration has nothing to do. Conclusions CK2α expression or activity increase is closely related to the occurrence of endometrial adenocarcinoma. CK2α may be a potential molecular target for the treatment of endometrial adenocarcinoma.