论文部分内容阅读
以菊花幼嫩花瓣作为供体材料，ＭＳ作为基本培养基，在激素组合为６ＢＡ１～３ｍｇ／Ｌ＋ＮＡＡ１ｍｇ／Ｌ或２，４Ｄ１～３ｍｇ／Ｌ＋ＫＴ１ｍｇ／Ｌ的范围内均能诱导出愈伤组织，但以ＭＳ附加６ＢＡ２ｍｇ／Ｌ和ＮＡＡ１ｍｇ／Ｌ的诱导率最高，可达１００％。将愈伤组织转移到ＭＳ＋６ＢＡ２ｍｇ／Ｌ＋ＮＡＡ０２ｍｇ／Ｌ的培养基上，分化芽的频率为７７８％。将３ｃｍ左右的茎段转移到不含激素的ＭＳ培养基或ＭＳ＋ＮＡＡ０１ｍｇ／Ｌ的培养基上，生根率可达９５％以上。
Using the young petals of chrysanthemum as the donor material and MS as the basic medium, the callus could be induced in the range of 6BA1 ~ 3mg / L + NAA1mg / L or 2,4D1 ~ 3mg / L + KT1mg / L with hormone combination, However, the highest induction rate of MS with 6BA2mg / L and NAA1mg / L was up to 100%. The callus was transferred to MS + 6BA2mg / L + NAA0.2mg / L medium, the frequency of bud differentiation was 77 8%. Will be about 3cm stems transferred to hormone-free MS medium or MS NAA0 1mg / L medium, the rooting rate of up to 95%.