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目的观察大黄素对急性角膜炎大鼠角膜组织中细胞间粘附分子-1(ICAM-1)表达的影响及意义。方法建立角膜炎大鼠模型,模型制备前30 min,大鼠结膜下分别注射大黄素(治疗组)及等容二甲基亚砜(炎症组)。按脂多糖(LPS)刺激后不同时点,各组又分为1、3、6、122、4 h组,每亚组8只鼠。于各时点观察大鼠的全身情况,裂隙灯显微镜观察大鼠结膜、角膜及房水等眼部表现;抽取大鼠房水涂片,HE染色观察房水细胞的变化;角膜组织切片,HE染色观察各组大鼠角膜的组织形态学改变;逆转录-聚合酶链反应(RT-PCR)检测大鼠角膜组织中ICAM-1 mRNA的表达。结果球结膜下预防性注射大黄素,可明显改善角膜炎大鼠畏光、流泪等眼部表现,减轻角膜混浊、组织水肿等炎症反应。大黄素预处理可明显减轻大鼠角膜组织及房水中炎症细胞浸润的程度,改善角膜组织水肿及角膜基质层胶原纤维排列的紊乱程度。LPS刺激可引起炎症组大鼠角膜组织中ICAM-1mRNA表达增加,该作用可被大黄素部分抑制,治疗组各时点ICAM-1 mRNA的表达较炎症组均明显降低(P均<0.01)。结论大黄素可减少急性角膜炎症反应中ICAM-1 mRNA的表达,对急性角膜炎大鼠角膜损伤起保护作用。
Objective To observe the effect and significance of emodin on the expression of intercellular adhesion molecule-1 (ICAM-1) in corneal tissue of rats with acute keratitis. Methods A rat model of keratitis was established. 30 min before the preparation of the model, the rats were injected with emodin (treated group) and isovolumic dimethyl sulfoxide (inflammation group) under the conjunctiva. After stimulation with lipopolysaccharide (LPS) at different time points, each group was divided into 1, 3, 6, 122, and 4 h groups, and each group had 8 rats. At each time point, the whole body of the rats was observed. The conjunctiva, cornea and aqueous humor of the rat were observed with slit lamp microscope. Atrial aqueous humor smears were taken from the rats, HE staining was used to observe the change of aqueous humor cells, and corneal tissue sections were obtained. The histological changes of the corneas of the rats in each group were observed by staining, and the expression of ICAM-1 mRNA in rat corneal tissues was detected by reverse transcription-polymerase chain reaction (RT-PCR). [Results] Preventive injection of emodin under the conjunctiva can significantly improve the ocular manifestations of photophobia and tearing in rats with keratitis, and reduce inflammatory reactions such as corneal opacity and tissue edema. Emodin pretreatment significantly reduced the degree of inflammatory cell infiltration in rat corneal tissue and aqueous humor, and improved corneal tissue edema and disorder of collagen fiber arrangement in corneal stroma. LPS stimulation could increase the expression of ICAM-1 mRNA in the corneal tissue of the inflammation group. This effect was partially inhibited by emodin. The expression of ICAM-1 mRNA in the treatment group was significantly lower than that in the inflammation group (P<0.01). Conclusion Emodin can reduce the expression of ICAM-1 mRNA in acute corneal inflammation and protect corneal injury in rats with acute keratitis.