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就棉铃虫田间种群对久效磷抗性的毒理学机制进行了研究。久效磷对敏感品系和高抗品系的乙酰胆碱酯酶抑制率存在显著差异,在同等剂量浓度下,对敏感品系的抑制率为73.48％,对高抗品系的抑制率仅为59.67％,说明棉铃虫对久效磷产生高水平抗性与乙酰胆碱酯酶不敏感性有关。在不同田间抗性品系中,PBO和TPP对久效磷均具有明显的增效作用,增效比分别为5.6～23.4倍和2.9～4.6倍;而相应地PBO和TPP对新疆敏感品系的增效比仅分别为1.3和1.7倍。6龄幼虫头部羧酸酯酶活力测定结果表明,在高抗品系中,羧酸酯酶活力为3.83×10~(-5)mmol α-NA/头.min,而敏感品系为2.18×10~(-5)mmol α-NA/头.min,二者差异显著。据此认为,多功能氧化酶和羧酸酯酶代谢作用的增强是棉铃虫对久效磷产生抗性的原因之一。
The toxicological mechanism of monocrotophos resistance in the cotton bollworm population was studied. The inhibitory rate of monocrotophos to acetylcholinesterase in sensitive and high resistant strains was significantly different. The inhibition rate of sensitive strains and susceptible strains was 73.48% and 59.67% at the same dosage, which indicated that the bolls Insect Monocrotophos produce high levels of resistance and acetylcholinesterase insensitivity. PBO and TPP had significant synergistic effects on monocrotophos in different field resistance lines, the efficiency-increasing ratios were 5.6 ~ 23.4 times and 2.9 ~ 4.6 times, respectively; while the PBO and TPP increased the susceptibility of Xinjiang Effective ratio was only 1.3 and 1.7 times. Carboxyl esterase activity of 6th instar larvae showed that carboxylesterase activity was 3.83 × 10 ~ (-5) mmol α-NA / head · min in the high resistant lines and 2.18 × 10 ~ (-5) mmol α-NA / head .min, the difference was significant. Therefore, it is considered that the enhancement of multifunctional oxidase and carboxylesterase metabolism is one of the reasons why cotton bollworm is resistant to monocrotophos.