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血清、精浆或宫颈粘液中存在抗精子抗体(ASA)时,可以引起不育。一般使用的ASA检测方法如明胶凝集试验(GAT),精子制动试验(SIV)及平板凝集试验(TAT)需要志愿者提供新鲜高质量的精液,并需有经验的技术操作。由于提供精液的人数有限且观察结果费时较长,故一天仅能测少量标本。作者使用一种改良酶联免疫吸附法(ELISA)即以带正电荷的聚氯乙烯(PVC)微量反应板代替聚苯乙烯板来吸附未经固定的精子,从而避免了由于聚苯乙烯板使用戊二醛结合精子而引起精子抗原性的变化。此法大致步骤如下:先将手淫取得的正常精液标本(精子数≥30×10~6sp/ml,活力≥40%,正常形态精子≥50%,无白细胞,无凝集现象)经PBS洗涤三次,制成精子悬
Anti-sperm antibodies (ASA) present in serum, seminal plasma or cervical mucus can cause infertility. The commonly used methods of ASA testing, such as gelatin agglutination test (GAT), sperm brake test (SIV), and plate agglutination test (TAT) require volunteers to provide fresh, high-quality semen that is subject to experienced technical procedures. Due to the limited number of people providing sperm and the long and time-consuming observation, only a small number of specimens can be measured in one day. The authors used a modified enzyme-linked immunosorbent assay (ELISA) to replace uncoated sperm with a polystyrene plate coated with a positively charged polyvinylchloride (PVC) microtiter plate, thereby avoiding the use of polystyrene plates Glutaraldehyde and sperm cause sperm antigenic changes. The general steps of this method are as follows: Firstly, the normal semen specimens obtained by masturbation (sperm count≥30 × 10 ~ 6sp / ml, vitality≥40%, normal morphology sperm≥50%, no white blood cells, no agglutination) Made of sperm suspended