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应用细胞培养法评价各种生物材料已有许多报道。目前正在成为一种有效的方法。细胞培养法试验中有多种方法。如琼脂重层法(agar overlay technique),细胞与生物材料直接接触法,放射性元素示踪法,显微镜细胞形态观察法,细胞增殖度测定法:①琼脂上细胞集落形成法,②细胞数及细胞核数计数法(包括血球计数板计数法及器械的面积指数计算法),③细胞混浊度测定法,④细胞生死的判定法等等。
There are many reports on the use of cell culture to evaluate various biological materials. It is becoming an effective method. There are many ways in cell culture assays. Such as agar overlay technique, direct contact between cells and biomaterials, radiolabeled tracer method, microscopic observation of cell morphology and cell proliferation assay: ① colony formation on agar, ② cell number and nucleus Counting method (including the counting method of blood count plate and the area index of the instrument method), ③ cell turbidimetry, ④ determine the law of cell death and so on.