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为探讨中药化痰散结方诱导人肺癌细胞 (SPC A1)凋亡的作用及其对正常人胚肺细胞的毒性作用 ,通过血清药理学方法 ,选择不同浓度的含药兔血清与人肺癌细胞共同孵育处理 ,并以正常人胚肺细胞 (HEL)作对照。结果 :人肺癌细胞经含中药兔血清作用 ,由贴壁生长而逐渐脱落 ,外形变圆 ,体积缩小 ,折光性增强 ;荧光镜下可见细胞核破碎 ,裂解为大小不等的凋亡小体 ;流式细胞仪检测显现典型的细胞凋亡峰 ,以 5%含药血清作用 4 8h凋亡峰最为明显 ,凋亡率为 4 2 .8% ;出现S期细胞阻滞 ;而该含药血清对人胚肺细胞无诱导凋亡作用。化痰散结方通过血清药理学方法诱导人肺癌细胞的凋亡 ,可能是其抗肿瘤的分子学机制之一。
To investigate the effect of Huayu Huayu Sanjiefang on the apoptosis of human lung cancer cells (SPC A1) and its toxic effects on normal human embryonic lung cells, different concentrations of drug-containing rabbit serum and human lung cancer cells were selected by serum pharmacology. The cells were co-incubated and normal human embryonic lung cells (HEL) served as controls. RESULTS: Human lung cancer cells were treated with rabbit serum containing traditional Chinese medicine and gradually fell off due to adherent growth. The shape of the human lung cancer cells was rounded, the volume was reduced, and the refractive index was enhanced. The nucleus was broken and lysed into apoptotic bodies of varying sizes under fluoroscopy; The typical apoptotic peaks were detected by cytometry. The most obvious apoptotic peak was 48 hours with 5% drug-containing serum. The apoptotic rate was 42.8%; S phase cell block occurred; Human embryonic lung cells did not induce apoptosis. Huayu Sanjie Fang induces apoptosis of human lung cancer cells by serum pharmacology, which may be one of its molecular mechanisms of anti-tumor.