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目的探讨麻黄素对小鼠心肌组织的毒性影响。方法 60只小鼠随机分为麻黄素组和对照组,每组各30只,用递增剂量腹腔连续注射麻黄素溶液(0.0315 g/kg、0.0477 g/kg、0.0564 g/kg)和等量的生理盐水15d(每天两次,每次0.2ml),称量检测小鼠体重和心重的变化,用生物显微技术观察心肌组织结构的变化,用比色法检测血浆肌酸激酶(CK)、肌酸激酶同工酶-MB(CK-MB)和心肌组织总抗氧化能力(T-AOC)、过氧化氢酶(CAT)活力及丙二醛(MDA)含量的变化。结果麻黄素组小鼠体重和心重均低于对照组,小鼠心肌组织出现不同程度的损伤,心肌细胞排列紊乱,闰盘间隙增宽,心肌纤维断裂;麻黄素组小鼠血浆CK、CK-MB活性高于对照组(P<0.01),心肌组织T-AOC和CAT的活性降低(P<0.05或P<0.01),MDA含量则升高(P<0.01)。结论麻黄素影响小鼠心肌组织结构,可能与心肌组织抗氧化酶活性降低和丙二醛含量升高有关。
Objective To investigate the toxicity of ephedrine on myocardium in mice. Methods Sixty mice were randomly divided into ephedrine group and control group, with 30 rats in each group. The mice were injected intraperitoneally with escalating doses of ephedrine solution (0.0315 g / kg, 0.0477 g / kg, 0.0564 g / kg) The rats were sacrificed and the rats were sacrificed. The rats were sacrificed and the rats were sacrificed. The rats were sacrificed and their weights were measured. (CK-MB) and total myocardial antioxidant capacity (T-AOC), catalase (CAT) activity and malondialdehyde (MDA) Results The body weight and heart weight of ephedrine group were lower than that of the control group. The myocardial tissue of the ephedrine group showed different degrees of injury, the myocardial cells were disordered, the intercalated disc gap widened and the myocardial fiber was broken. The plasma levels of CK, CK- (P <0.01 or P <0.01). The activities of T-AOC and CAT in myocardium were decreased (P <0.05 or P <0.01) and MDA content was increased (P <0.01). Conclusion Ephedrine can affect the structure of myocardium in mice and may be related to the decrease of myocardial antioxidant enzyme activity and the increase of malondialdehyde content.