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ERF转录因子广泛存在于植物中并且参与植物应对生物及非生物胁迫的响应。本研究利用RT-PCR技术从大豆中克隆获得1个新的ERF转录因子基因Gm ERF8,开放阅读框全长627 bp,编码1个由208个氨基酸残基组成的分子量为23.43 k D的蛋白。蛋白结构预测发现,该蛋白含有1个典型的AP2/ERF结合域,2个预测的核定位信号和1个保守的EAR抑制元件。进化分析表明Gm ERF8蛋白与烟草Nt ERF3蛋白的同源性最高。实时荧光定量PCR表明,Gm ERF8在大豆的根和叶中表达量较高。ABA、高盐和低温处理均使Gm ERF8表达量下降;乙烯(ET)和干旱处理则使Gm ERF8的表达量先下降后升高。转录调节能力分析结果显示,Gm ERF8可以抑制报告基因的表达。上述实验结果表明,Gm ERF8可能作为转录抑制子参与大豆对环境胁迫的应答。
ERF transcription factors are widespread in plants and are involved in the response of plants to both biological and abiotic stresses. In this study, a new ERF transcription factor gene Gm ERF8 was cloned from soybean by RT-PCR. The full-length open reading frame of Gm ERF8 is 627 bp in length and encodes a protein with a molecular weight of 23.43 kD consisting of 208 amino acid residues. Protein structure prediction revealed that this protein contains one typical AP2 / ERF binding domain, two predicted nuclear localization signals and one conserved EAR inhibitor. Phylogenetic analysis showed that Gm ERF8 protein had the highest homology with tobacco Nt ERF3 protein. Real-time PCR showed that Gm ERF8 was highly expressed in roots and leaves of soybean. ABA, high salt and low temperature treatments both decreased the expression of Gm ERF8; ethylene (ET) and drought treatment decreased the expression level of Gm ERF8 first and then increased. Transcriptional regulatory ability analysis showed that Gm ERF8 can inhibit the expression of the reporter gene. The above experimental results show that Gm ERF8 may participate in the response of soybean to environmental stress as a transcriptional repressor.