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[目的]通过研究原发性胃腺癌8号染色体倍性改变,对比胃癌与癌旁组织的差异,探讨其与胃癌病理诊断及临床病理指标的相关性。[方法]选取文献报道的胃癌中非整倍体出现频率较高的8号染色体的着丝粒探针,应用荧光原位杂交技术对127例胃腺癌、32例癌旁组织及5例远端胃黏膜组织进行了检测。复阅相应癌和癌旁组织病理切片和临床病理资料,分析所测样本的非整倍体发生率及其临床相关性。[结果]胃癌组织的8号染色体非整倍体发生率为68.5%,显著高于癌旁组织(15.6%),具有统计学差异(P=0.000)。8号染色体倍体改变与患者性别、年龄、部位、大体分型、肿瘤大小、浸润深度、脉管瘤栓、淋巴结转移及病理TNM分期均无显著相关性(P>0.05)。5例癌旁组织检测出8号染色体非整倍体(三体),病理组织学改变为轻度慢性炎症,其中2例伴有轻度肠上皮化生。远端胃黏膜组织8号染色体均表现为正常倍体数(二体)。[结论]8号染色体倍体改变为胃癌的诊断提供了候选分子细胞遗传学指标,可能是胃癌发生的早期遗传学改变之一,对早期诊断具有一定提示意义。
[Objective] To study the difference between gastric cancer and paracancerous tissue by studying the ploidy change of chromosome 8 in primary gastric adenocarcinoma, and to explore its correlation with pathological diagnosis and clinicopathological parameters. [Methods] Centromeric probes of chromosome 8 in aneuploidy of gastric cancer, which reported in the literature, were used for the detection of 127 cases of gastric adenocarcinoma, 32 cases of paracancerous tissues and 5 cases of distal end by using fluorescence in situ hybridization Gastric mucosal tissue was tested. Review the corresponding cancer and paracancerous tissue biopsy and clinicopathological data, analyze the aneuploidy incidence of the sample and its clinical relevance. [Results] The incidence of chromosome 8 aneuploidy in gastric cancer was 68.5%, which was significantly higher than that in paracancerous tissues (15.6%) with statistical significance (P = 0.000). There was no significant correlation between the ploidy change of chromosome 8 and the gender, age, location, general classification, tumor size, depth of invasion, tumor thrombus, lymph node metastasis and TNM stage (P> 0.05). 5 cases of paracancerous tissues detected chromosome 8 aneuploidy (trisomy), pathological changes to mild chronic inflammation, of which 2 cases associated with mild intestinal metaplasia. Distal gastric mucosa chromosome 8 showed normal ploidy (two body). [Conclusion] The change of ploidy on chromosome 8 provides candidate molecular cytogenetic markers for the diagnosis of gastric cancer, which may be one of the early genetic changes of gastric cancer, which may be of some indication to early diagnosis.