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目的 研究共刺激途径Ｂ７／ＣＤ２８ 和ＩＣＡＭ１／ＬＦＡ１ 对Ｔ 细胞活化以及Ｂ 细胞效应的作用。方法 在体外建立ＡＰＣＴＢ 细胞反应系统， 用Ｂ７１ 单抗和ＩＣＡＭ１ 单抗分别阻断Ｂ７／ＣＤ２８ 和ＩＣＡＭ１／ＬＦＡ１ 共刺激途径， 利用３ ＨＴｄＲ 法检测Ｔ 细胞增殖，ＥＬＩＳＡ 法测定Ｂ 细胞分泌的抗体， 用ＲＴＰＣＲ 法检测细胞因子基因的表达。结果 Ｂ７１ 单抗和ＩＣＡＭ１ 单抗均可抑制Ｔ 细胞增殖及ＩＬ２ 的产生。Ｂ７１ 单抗可下调Ｂ 细胞抗体的产生（ Ｐ＜ ０ ．０５） ， 而ＩＣＡＭ１ 单抗未见明显的抑制（ Ｐ＞ ０ ．０５） 。Ｂ７１ 单抗和ＣｓＡ 联用能阻断Ｔ 细胞增殖活性及Ｂ 细胞的效应， 而ＩＣＡＭ１ 单抗和ＣｓＡ联用则无此作用。Ｂ７１ 单抗能下调ＩＬ２ 和ＩＦＮγｍ ＲＮＡ 表达，Ｂ７１ 单抗和ＣｓＡ 联用则阻断ＩＬ２ 和ＩＦＮγｍ ＲＮＡ 表达，ＩＬ４ 和ＩＬ１０ ｍ ＲＮＡ 仍可表达。结论 Ｂ７／ＣＤ２８ 和ＩＣＡＭ１／ＬＦＡ１ 共刺激途径在Ｔ 细胞活化中具有不同的作用，Ｂ７１ 单抗和ＣｓＡ 联用可导致Ｔ 细胞功能失活即无能。
Objective To study the effects of costimulatory pathway B7 / CD28 and ICAM1 / LFA1 on T cell activation and B cell response. Methods APC TB cell response system was established in vitro. The co-stimulatory pathway of B7 / CD28 and ICAM1 / LFA1 was blocked by B71 monoclonal antibody and ICAM1 monoclonal antibody respectively. The 3 HTdR assay T cell proliferation, ELISA assay of B cell secretion of antibodies, RT-PCR assay cytokine gene expression. Results B7 1 monoclonal antibody and ICAM 1 monoclonal antibody can inhibit T cell proliferation and IL 2 production. B7 1 monoclonal antibody can down-regulate the production of B cell antibodies (P <0 .05), while ICAM 1 monoclonal antibody showed no significant inhibition (P> 0.05). B7 1 monoclonal antibody and CsA can block the proliferation of T cell activity and B cell effects, and ICAM 1 monoclonal antibody and CsA combined with no such effect. B7 1 monoclonal antibody can be reduced IL 2 and IFN γ m RNA expression, B7 1 monoclonal antibody and CsA combined with blocking IL 2 and IFN γ m RNA expression, IL 4 and IL 10 m RNA can still expression. Conclusion B7 / CD28 and ICAM 1 / LFA 1 costimulatory pathway in T cell activation has a different role, B7 1 monoclonal antibody and CsA can lead to T cell dysfunction that is incompetent.