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目的观察分析死后不同时间冷冻大鼠尸体主要内脏组织细胞形态变化规律。方法 SD大鼠20只,分为尸僵前冷冻组、尸僵期冷冻组、尸僵缓解冷冻组及对照组(各5只),颈椎脱臼处死大鼠,实验组大鼠在室温25℃±5℃、相对湿度80%±5%分别放置0h、6h、24h后-20℃±2℃冰箱冷冻72h,解剖提取脑、心、肺、肝、肾,常规切片HE染色,显微图像体视学测量细胞外间隙的面密度。结果各实验组内脏组织间质疏松增宽,冰晶裂隙沿组织间隙或肝血窦分布、周边细胞固缩,红细胞冻溶、血管周围浆液淤积,以肝组织变化最明显。细胞外间隙的面密度:对照组明显小于实验组(P<0.01);脑尸僵前组较尸僵组大(P<0.05);心、肾尸僵前组和尸僵组无显著差异(P>0.05),均较尸僵缓解组大(P<0.01);肝各实验组组间比较均无显著差异(P>0.05)。结论冷冻不同尸僵状态下内脏组织细胞有不同的形态结构,可作为区别和判断生前和死后组织细胞形态改变的参考。
Objective To observe and analyze the morphological changes of major visceral cells in frozen cadaver of rats after death at different time points. Methods Twenty SD rats were divided into two groups: frozen before necrosis group, frozen necrosis group, frozen necrosis group and control group (5 rats each). Rats were sacrificed by cervical dislocation. Rats in experimental group were stored at 25 ℃ ± 5 ℃, relative humidity 80% ± 5% were placed 0h, 6h, 24h after freezing at -20 ℃ ± 2 ℃ refrigerator 72h, anatomical extraction of brain, heart, lung, liver, kidney, conventional sections HE staining, Measure the areal density of extracellular space. Results The interstitial loosening of visceral tissue in each experimental group was broadened. The distribution of ice crystal fissure along the interstitial space or hepatic sinusoids, the shrinkage of surrounding cells, the lyophilization of erythrocytes, and the deposition of serous fluid around the perivascular tissue were the most obvious changes in liver tissues. The density of extracellular space was significantly lower in the control group than that in the experimental group (P <0.01), that in the anterior dead group was higher than that in the dead group (P <0.05) P> 0.05), which were significantly higher than those in the group treated with necrosis (P <0.01). There was no significant difference between the experimental groups (P> 0.05). Conclusions There are different morphological structures of visceral cells frozen in different dead bodies, which can be used as a reference to distinguish and determine the morphological changes of the cells before and after death.