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辣根过氧化物酶(HRP)是目前在ELISA中应用最普遍的标记酶。但是目前常用的显色底物,如 5氨基水杨酸、邻联茴香胺、邻联甲苯胺或邻苯二胺(OPD)等都被认为是致癌物质。寻找适用于ELIS人的非致癌性HRP显色底物有着重要的实际意义。磺基二氯苯酚(SDP)和4-氨基安替比林(AAP)经HRP催化氧化后结合成玫瑰红色化合物,这一反应曾被用于血液尿酸测定,即测定通过尿酸酶作用于尿酸而释放的H_2O_2。但在该反应中H_2O_2最适浓度极低,过量的H_2O_2对HRP产生强
Horseradish peroxidase (HRP) is the most commonly used marker enzyme in ELISA. However, commonly used chromogenic substrates such as 5-aminosalicylic acid, o-dianisidine, o-tolidine or o-phenylenediamine (OPD) are all considered carcinogens. It is of great practical significance to find a non-carcinogenic HRP chromogenic substrate for ELIS human. Sulfodichlorophenol (SDP) and 4-aminoantipyrine (AAP) catalyzed oxidation by HRP bound to a rose-red compound, a reaction that has been used for the determination of blood uric acid, ie the determination of uricase by uricase Release of H_2O_2. However, the optimum concentration of H 2 O 2 in this reaction was extremely low, and the excess H 2 O 2 produced strong HRP