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背景:睫状神经营养因子(CNTF)对失神经支配骨骼肌萎缩有防治作用,但是否可以应用CNTF基因转染方法延缓失神经支配骨骼肌萎缩尚不清楚。目的:探索一次性电穿孔介导CNTF转染延缓失神经骨骼肌萎缩的疗效。设计:随机对照研究。地点和对象:在上海交通大学附属第一人民医院动物房完成,雄性SD大鼠36只,年龄两三个月,体质量250～300g,由上海中科院实验动物中心提供。干预:制备36只大鼠右下肢腓肠肌失神经支配模型,随机平均分成失神经对照组,CNTF基因转染组。两组于术后2,4,8周分别取6只大鼠进行实验评价。主要观察指标:两组术后2,4,8周肌湿重维持率、肌细胞截面积、肌肉蛋白含量、胶原纤维与肌细胞面积比和细胞凋亡数。结果:2周和4周CNTF基因转染组的肌湿重维持率、肌细胞截面积和肌肉蛋白含量均明显高于失神经对照组(P<0.05),而胶原纤维与肌细胞面积比和细胞凋亡数明显低于失神经对照组(P<0.05)。8周后,两组间各参数无明显区别(P>0.05)。结论:一次性电穿孔介导CNTF基因转染可延缓失神经骨骼肌萎缩4周。
BACKGROUND: Ciliary neurotrophic factor (CNTF) can prevent and treat denervation of skeletal muscle atrophy. However, it is unclear whether CNTF gene transfection can delay denervation and skeletal muscle atrophy. OBJECTIVE: To investigate the effect of one-time electroporation mediated CNTF transfection on denervated skeletal muscle atrophy. Design: Randomized controlled study. Location and Subjects: At the Animal Hospital of the First People’s Hospital Affiliated to Shanghai Jiaotong University, 36 male SD rats, aged two to three months with a body weight of 250-300 g, were provided by the Experimental Animal Center of Shanghai Chinese Academy of Sciences. Intervention: The denervated model of gastrocnemius in the right lower limb of 36 rats was randomly divided into control group and CNTF gene transfected group. Two groups of rats were evaluated at 2,4,8 weeks after operation respectively. MAIN OUTCOME MEASURES: Muscle weight maintenance rate, muscle cell cross-sectional area, muscle protein content, collagen fiber to muscle cell area ratio and cell apoptosis number in two groups at 2, 4, and 8 weeks after operation. Results: After 2 weeks and 4 weeks, the muscle wet weight maintenance, muscle cell cross-sectional area and muscle protein content in CNTF gene transfected group were significantly higher than those in denervated group (P <0.05), while the ratio of collagen fiber to muscle cell area The number of apoptosis was significantly lower than that of the control group (P <0.05). After 8 weeks, there was no significant difference between the two groups (P> 0.05). Conclusion: One-time electroporation mediated CNTF gene transfection can delay denervation skeletal muscle atrophy for 4 weeks.