目的:为解决替代对照品法或一测多评法在相对校正因子法定量和相对保留时间法定性方面的误差有时偏大的问题,建立了一种新的替代对照品模式的多指标含量测定法—双标多测法。方法:在3台不同品牌的高效液相色谱仪和23种不同品牌和型号的C18色谱柱上,测定重楼中4个皂苷类成分的峰面积和保留时间,计算相应的相对校正因子和相对保留时间,再进行数据偏差的比较和分析。结果:定量方面,解释了检测波长和仪器波长偏移影响相对校正因子的机制,并据此提出了波长选择建议;定性方面,证明了梯度条件下,双标线性校正法预测保留时间的效果仍明显优于相对保留时间法。结论:双标多测法在中药多指标含量测定方面具有较好的实用价值和较广的应用前景。 OBJECTIVE: In order to solve the problem that the relative error of the calibration factor method and the relative retention time are sometimes too large, a new alternative reference substance model for the determination of multiple indexes Law - double standard and more test. Methods: The peak area and retention time of four saponins were determined in three different brands of HPLC and 23 C18 columns of different brands and models, and the corresponding relative correction factors and relative Retention time, then data deviation comparison and analysis. Results: Quantitatively, the mechanism of the relative correction factor of the wavelength and the wavelength shift of the instrument was explained, and the wavelength selection suggestion was put forward accordingly. On the qualitative aspect, the effect of the double standard linear calibration method on the retention time was proved under the gradient condition Obviously better than the relative retention time method. Conclusion: The double standard and multiple test methods have good practical value and broad application prospects in the determination of multi-index content of traditional Chinese medicine.