目的探讨半夏白术天麻汤治疗痰湿壅盛型高血压病的可能分子机制。方法大鼠高脂饮食24周建立痰湿壅盛型高血压模型,16只造模成功大鼠随机分为模型组和半夏白术天麻汤组,每组各8只,并以普通饲料喂养大鼠8只设为正常组。半夏白术天麻汤组给予半夏白术天麻汤13.8 g/(kg·d)灌胃,正常组和模型组给予等量生理盐水灌胃,均灌胃12周。记录大鼠体重、血压,计算Lee’s指数,检测大鼠血清葡萄糖、甘油三酯、总胆固醇水平;双向电泳分离样品并且进行硝酸银染色,筛选下丘脑差异蛋白质点提交质谱鉴定,采用MALDI-TOF-TOF质谱仪进行质谱分析、肽指纹图谱进行蛋白质鉴定,选取部分蛋白质点验证蛋白质组学结果。结果半夏白术天麻汤组大鼠体重、血压、Lee’s指数、血清总胆固醇均较模型组下降(P<0.05)。正常组、模型组、半夏白术天麻汤组分别分离出3342、3844、3828个蛋白点,其中半夏白术天麻汤组同模型组比较,100个蛋白质表达降低,72个蛋白质表达增加。在质谱仪进一步鉴定的19种蛋白中,模型组与正常组相比有差异而半夏白术天麻汤可使差异趋势逆转的蛋白点为11个,其中5个表达上调,6个表达下调。结论半夏白术天麻汤治疗痰湿壅盛型高血压病的分子机制可能与改善细胞骨架和形态、内质网应激、能量代谢、神经元结构等有关。 Objective To investigate the possible molecular mechanism of Banxia Baizhu Tianma Decoction in treating phlegm-dampness-type hypertension. Methods Rats with high-fat diet were given phlegm-dampness model of hypertension for 16 weeks. Sixteen successful rats were randomly divided into model group and Banxia Baizhu Tianma Decoction group, with 8 rats in each group and fed with normal diet 8 rats as normal group. Pinellia Atractylodes Tianma Decoction group was given Banxiabaizhu Tianma Decoction 13.8 g / (kg · d) gavage, the normal group and model group were given the same amount of saline gavage, were gavage for 12 weeks. The weight and blood pressure of rats were recorded, and the Lee’s index was calculated. The levels of serum glucose, triglyceride and total cholesterol in rats were determined. The samples were separated by two-dimensional electrophoresis and stained with silver nitrate for screening the differential protein spots in the hypothalamus for mass spectrometry identification. MALDI-TOF- TOF mass spectrometry mass spectrometry, peptide fingerprinting protein identification, selected part of the protein spots validation proteomics results. Results The body weight, blood pressure, Lee’s index and serum total cholesterol in Banxia Baizhu Tianma Decoction group were lower than those in model group (P <0.05). There were 3342,3844 and 3828 protein spots in normal group, model group and Banxiabaizhu Tianma Decoction group, respectively. Among them, compared with model group, Banxiabaizhu Tianma Decoction group reduced 100 protein expression and 72 protein expression increased. Of the 19 proteins further identified by mass spectrometry, there were differences between the model group and the normal group. 11 spots were found in Banxia Tianzhu Tianma Decoction, of which 5 were up-regulated and 6 were down-regulated. Conclusion The molecular mechanism of Banxiabaizhu Tianma Decoction in treating phlegm-dampness-type hypertension may be related to the improvement of cytoskeleton and morphology, endoplasmic reticulum stress, energy metabolism and neuronal structure.