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对转DREB1A基因多年生黑麦草(Lolium perenne)的T1代种子和多年生黑麦草非转化植株的子一代种子进行了不同质量分数的NaCl(0、0.3%、0.6%、0.9%、1.2%、1.5%)处理,研究了转基因植株对盐胁迫的生理响应。结果表明,随着盐质量分数递增,相对发芽势、相对发芽率、胚芽长、叶绿素含量、叶片含水量及相对含水量呈现下降的趋势,转基因组与对照组之间的差异达到显著水平,高盐胁迫下,前者各指标均高于后者;胚根长变化无明显规律;细胞膜透性的变化与盐质量分数的增加成正相关,转基因组的细胞膜透性低于对照组,二者存在显著差异。说明转基因植株的耐盐性明显强于对照组,转DREB1A基因多年生黑麦草T1代种子可以作为盐害土壤改良和利用的植物资源。
The seeds of T1 progeny from perennial ryegrass (Lolium perenne) and non-transformed perennial ryegrass from DREB1A gene were treated with different mass fractions of NaCl (0, 0.3%, 0.6%, 0.9%, 1.2%, 1.5% ) To study the physiological response of transgenic plants to salt stress. The results showed that relative germination potential, relative germination rate, germination length, chlorophyll content, leaf water content and relative water content tended to decrease with the increasing of salt mass fraction, and the difference between transgenic group and control group reached significant level Under salt stress, the indexes of the former were higher than those of the latter; the change of radicle length had no obvious regularity; the change of cell membrane permeability was positively correlated with the increase of salt mass fraction; the membrane permeability of the transgenic group was lower than that of the control group difference. The results showed that the salt tolerance of transgenic plants was significantly stronger than that of the control. The T1 seeds of perennial ryegrass transferred with DREB1A gene could be used as plant resources for salt improvement and utilization.