以金山57×金山630的杂交后代所得的F1分离群体为材料,按F1单株抗性分群,建立抗病池和感病池,分别以其为摸板进行RAPD分析,以获得差异片段。应用RAPD技术筛选了320个随机引物,其中仅1个引物即S213-500在抗感池间扩增出了多态性谱带。应用结果表明该标记与田间鉴定的吻合率较高,能得到重复性验证和可靠的差异性扩增片段,可以作为抗I型薯瘟基因的连锁标记。 The F1 segregation population derived from the cross of offspring of Jinshan 57 × Jinshan 630 was used as materials, and the disease resistant pool and susceptible pool were established according to the resistance of F1 plants. Respectively, RAPD analysis was carried out using this as a template to obtain the difference fragments. Three hundred and seventy random primers were screened by RAPD technique. Among them, only one primer, S213-500, amplified the polymorphic bands in the anti-sense pool. The results showed that the marker was highly consistent with the field identification and could be used as a marker for the identification of the anti-type I blast crisis gene by obtaining reproducible and reliable differential amplified fragments.