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目的 探讨乳腺导管上皮非典型增生 (AIDH)恶变潜能的量化评估方法。方法 :应用自动图像分析系统对正常乳腺组织 10例 (正常组 ,NB)、乳腺导管上皮单纯性增生 33例 (增生组 ,IDH )、非典型增生 2 7例 (非典组 ,AIDH)和浸润性导管癌 37例 (导管癌组 ,IDCa)进行细胞DNA含量 (DI)及倍体分析、细胞核形态学计量。结果 1.在NB、IDH、AIDH和IDCa各组 ,DI及倍体分析参数 3- 4c %、5c %、>5c %均依次显著增高 (P均 <0 .0 1) ,2c %依次显著降低 (P <0 .0 1) ;核形态学参数 [核面积 (ar)、核周长 (ci)、核长径 (dl)、核短径 (ds)、核形状因子 (df) ]均依次显著增加 (P均<0 .0 1)。除 3- 4c %外 ,两类参数在非典组与导管癌组间有显著差异 (P <0 .0 5或P <0 .0 1)。 2 .ar、ci、dl、ds和df分别与DI、3- 4c %、5c %、>5c %呈显著正相关 ,与 2c %呈显著负相关。 3.根据图像分析ar、df、DI、2c %、5c %和 >5c % 6项指标的计量结果 ,建立乳腺导管上皮非典型增生和导管癌的判别方程 :Ya =- 2 76 .148+ 0 .12 3ar + 4 96 .832df + 10 9.330DI + 1.50 1(2c % ) - 0 .4 59(5c % )- 2 .2 87(>5c % ) ;Yc =- 2 82 .12 6 + 0 .154ar + 4 99.6 4 1df + 111.753DI + 1.4 2 3(2c % ) - 0 .2 2 1(5c % ) - 2 .36 7(>5c % )结论 ?
Objective To investigate the quantitative evaluation of the malignant potential of breast ductal atypical hyperplasia (AIDH). Methods: 10 cases of normal breast tissue (normal group, NB), 33 cases of proliferative breast ductal hyperplasia (proliferative group, IDH), 27 cases of atypical hyperplasia (Atypical group, AIDH) and infiltrative 37 cases of ductal carcinoma (ductal carcinoma group, IDCa) DNA content (DI) and ploidy analysis, nuclear morphology measurement. RESULTS: In the NB, IDH, AIDH and IDCa groups, the DI and the ploidy analysis parameters were significantly increased (P <0.01), followed by 2c% (P <0. 01). The nuclear morphological parameters (ar, ci, dl, d, df) Significantly increased (P <0.01). Except for 3- 4c%, there were significant differences in the two types of parameters between the group of SARS and that of the ductal carcinoma (P <0.05 or P <0.01). 2.ar, ci, dl, ds and df were significantly and positively correlated with DI, 3-4c%, 5c% and> 5c% respectively, but negatively correlated with 2c%. According to the measurement results of six indicators of ar, df, DI, 2c%, 5c% and> 5c%, the discriminant equation of atypical hyperplasia and ductal carcinoma of the mammary ductal epithelium was established: Ya = - 2 76 .148 + 0 .12 3ar + 4 96 .832df + 10 9.330DI + 1.50 1 (2c%) - 0.499 (5c%) - 2.2.77 (> 5c%); Yc = -282.126 + 0. 154ar + 4 99.6 4 1df + 111.753 DI + 1.4 2 3 (2c%) - 0 .2 2 1 (5c%) - 2.36 7 (> 5c%) Conclusion?