[目的 ]研究肝脏的细胞色素 P45 0 (CYP45 0 )的含量 ,反映 CYP2 C活性的氨基比林 - N-去甲基化酶(ANDM)及谷胱苷肽 - S-转移酶 (GST)的活性水平在诱导原发性肝癌过程中的变化。 [方法 ]用观察肝脏的 CYP45 0的含量、二甲基氨基偶氮苯 (DAB)作为诱导剂 ,喂养 Wistar雄性大鼠 ,ANDM和 GST的活性水平。 [结果 ]与对照组相比 ,诱癌组 CYP45 0含量及 GST、 ANDM的活性在 2周时无显著性差异 ;CYP45 0含量及 ANDM的活性在第 12、 30周时明显降低 ,GST在 12周时明显增高 ,而在 30周时与对照组无差别。在形成肝癌的大鼠肝脏中 ,肝组织中的 CYP45 0含量水平依次是 :癌组织 <癌旁组织 <正常组织。 [结论 ]DAB诱导的原发性大鼠肝癌形成过程中肝脏的 CYP45 0的含量有下降的趋势 ,DAB不能诱导 ANDM活性 ,但能诱导增强 GST的活性水平。 [Objective] To investigate the effects of aminopyrine - N - demethylase (ANDM) and glutathione - S - transferase (GST) in liver on the content of cytochrome P45 0 (CYP45 0) Changes of activity in the induction of primary liver cancer. [Methods] The activity levels of ANDM and GST in male Wistar rats were observed by observing the content of CYP45 0 in the liver and dimethylaminoazobenzene (DAB) as inducer. [Results] Compared with the control group, CYP45 0 content and the activities of GST and ANDM in the cancer-inducing group had no significant difference at 2 weeks; the content of CYP45 0 and the activity of ANDM in the 12th and 30th week significantly decreased, while the GST was 12 Week was significantly higher, but at 30 weeks no difference with the control group. In the formation of liver cancer in rat liver, liver tissue CYP45 0 levels were: cancer tissue