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目的分析大鼠灌胃白头翁中白头翁皂苷B7(齐墩果酸3-α-L-吡喃鼠李糖基(1→2)[β-D-吡喃葡萄糖基(1→4)]-α-L-吡喃阿拉伯糖苷)后血浆、胆汁、尿液和粪便中的代谢产物及其体外肝微粒体孵育体系中的代谢产物。方法分别采集大鼠灌胃后的血液、尿液、粪便、胆汁以及体外肝微粒体孵育样品并制备样品,采用超高效液相色谱与飞行时间质谱联用(UHPLCQ-TOF-MS)技术和Triple TOFTM5600+高分辨率质谱自带的软件联合的方法分析、鉴定代谢产物的化学结构。结果在大鼠胆汁、尿、粪、血浆以及肝微粒体孵育体系中共检测到23种代谢产物,其中体外肝微粒体孵育体系中的代谢产物有12种。结论白头翁皂苷B7在大鼠体内外均以脱糖代谢、葡萄糖醛酸化代谢和羟基化为主,白头翁皂苷B7易被肝微粒体代谢转化而产生多种代谢物。
Objective To investigate the effect of Pulsatilla saponin B7 (oleanolic acid 3-α-L-rhamnopyranosyl (1 → 2) [β-D-glucopyranosyl -L-arabinopyranoside) metabolites in plasma, bile, urine and feces and their metabolites in an in vitro liver microsomal incubation system. Methods The samples of blood, urine, feces, bile and in vitro liver microsomes after gavage were collected, and the samples were prepared and analyzed by UHPLCQ-TOF-MS and Triple TOFTM5600 + high-resolution mass spectrometry comes with a software method of joint analysis to identify the chemical structure of metabolites. Results Twenty-three metabolites were detected in the rat bile, urine, feces, plasma and liver microsomal incubation system, of which 12 were metabolites in the in vitro liver microsomal incubation system. Conclusion Pulsatilla saponin B7 is mainly de-glycosylated, glucuronidated and hydroxylated in vivo and in vivo. Pulsatilla saponin B7 is easily metabolized by liver microsomes to produce a variety of metabolites.