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目的研究4种细胞毒素(CTX-10、CTX-11、CTX-12和CTX-13)对膀胱癌细胞株(BIU-87和EJ)的抑制增殖作用,筛选出活性最强的一种CTX与膀胱癌单克隆抗体(BDI-1)构建成免疫毒素(IT),并研究IT的体外抑制膀胱癌细胞株增殖的活性.方法用结晶紫法和[3H]-TdR掺入法检测CTXs对膀胱癌细胞株的
Objective To investigate the inhibitory effect of four cytotoxins (CTX-10, CTX-11, CTX-12 and CTX-13) on the proliferation of bladder cancer cell lines (BIU-87 and EJ) (BDI-1) was constructed into immunotoxin (IT) and the activity of IT in inhibiting the proliferation of bladder cancer cell lines in vitro was studied.Methods CTXs were detected by crystal violet staining and [3H] -TdR incorporation assay in bladder Of cancer cell lines