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本文报告了用同种抗原作补体结合反应诊断内脏利什曼病的效果。抗原系用从肯尼亚黑热病人分离的杜氏利什曼原虫的前鞭毛体制成。前鞭毛体经于4℃以900g离心20分钟,以盐水洗3次,并稀释成20倍的悬液。超声处理后,以32g离心1小时。测定上清液中的蛋白浓度为5mg/ml~(-1)。补体结合反应用Staak等(1979)改良的微量板进行。所用稀释液均为有镁钙离子的巴比妥缓冲液。绵羊红细胞按1:1的比例加Alsever液保存,在6~14天应用,用前用巴比妥缓冲液洗3次,并制成2%的红细胞悬液。
This article reports the effect of alloantigen as complement fixation in the diagnosis of visceral leishmaniasis. Antigen was made from promastigotes of Leishmania donovani isolated from kenyan kenya. Promastigotes were centrifuged at 900g for 20 minutes at 4 ° C, washed three times with saline and diluted to 20-fold suspensions. After sonication, centrifuge at 32g for 1 hour. The concentration of protein in the supernatant was determined to be 5 mg / ml -1. Complement binding reactions were performed using modified microplates of Staak et al. (1979). All dilutions used are barbiturates with magnesium-calcium ions. Sheep red blood cells by 1: 1 ratio plus Alsever fluid preservation, in 6 to 14 days of application, before use with barbiturate buffer wash three times, and made of 2% of the red blood cell suspension.