【摘 要】
：
The sperm nucleus is prone to sustain DNA damage before and after ejaculation.Distribution of the damage is not homogeneous,and the factors determining differential sensitivity among nuclear regions have not yet been characterized.Human sperm chromatin co
【机 构】
：
Department of Molecular Biology, Faculty of Biology, Universidad de León, Campus de Vegazana s/n Leó
论文部分内容阅读
The sperm nucleus is prone to sustain DNA damage before and after ejaculation.Distribution of the damage is not homogeneous,and the factors determining differential sensitivity among nuclear regions have not yet been characterized.Human sperm chromatin contains three structural domains,two of which are considered the most susceptible to DNA damage:the histone bound domain,harboring developmental related genes,and the domain associated with nuclear matrix proteins.Using a quantitative polymerase chain reaction (qPCR) approach,we analyzed the number of lesions in genes homeobox A3 (HOXA3),homeobox B5 (HOXB5),sex-determining region Y (SRY)-box 2 (SOX2),β-GLOBIN,rDNA 18S,and rDNA 28S in human sperm after ultraviolet irradiation (400 μW cm-2,10 min),H2O2 treatment (250 mmol I-1,20 min),and cryopreservation,which showed differential susceptibility to genetic damage.Differential vulnerability is dependent on the genotoxic agent and independent of the sperm nuclear proteins to which the chromatin is bound and of accessibility to the transcription machinery.Immunodetection of 8-hydroxy-2\'-deoxyguanosine (8-OHdG) showed that the highest level of oxidation was observed after H202 treatment.The distribution of oxidative lesions also differed depending on the genotoxic agent.8-OHdG did not colocalize either with histone 3 (H3) or with type Ⅱα + β topoisomerase (TOPO Ⅱα + β) after H2O2 treatment but matched perfectly with peroxiredoxin 6 (PRDX6),which is involved in H2O2 metabolism.Our study reveals that the characteristics of the sperm head domains are responsible for access of the genotoxicants and cause differential degree of damage to nuclear areas,whereas chromatin packaging has a very limited relevance.The histone-enriched genes analyzed cannot be used as biomarkers of oxidative DNA damage.
其他文献
This study aimed to investigate the functional and morphological changes in the corpus cavernosum after cavernous nerve (CN)injury or neurectomy and then reveal whether treatment with the angiotensin Ⅱ Type 1 receptor antagonist Iosartan would improve ere
The syndrome of multiple morphological abnormalities of the sperm flagella (MMAF) is a specific kind of asthenoteratozoospermia with a mosaic of flagellar morphological abnormalities (absent,short,bent,coiled,and irregular flagella).MMAF was proposed in 2
ARAMIS is an international Phase Ⅲ trial demonstrating the beneficial role of darolutamide,a novel anti-androgen that has been found to prolong metastasisfree survival in men with nonmetastatic castration-resistant prostate cancer.Darolutamide is a novel
Structural alterations in fibroelastic components of the penile corpus cavernousum (CC) may impair its compliance,resulting in venous leakage and erectile dysfunction (ED).Our study evaluated the effectiveness of noninvasive two-dimensional shear-wave ela
For patients with moderate-to-severe erectile dysfunction,implantable penile prostheses continue to be a viable treatment.Medical device developers apply design controls during the development cycle to ensure that a product performs as intended in the fin
Up to 15％ of male infertility has an immunological origin,either due to repetitive infections or to autoimmune responses mainly affecting the epididymis,prostate,and testis.Clinical observations and epidemiological data clearly contradict the idea that th
Urologists perform the majority of vasectomies in the United States;however,family medicine physicians (FMPs) perform up to 35％.We hypothesized that differences exist in practice patterns and outcomes between urologists and FMPs.Patients who underwent a v
Pubertal development may be altered in boys with cryptorchidism and hypospadias,but existing knowledge is inconsistent.Therefore,we investigated the association between cryptorchidism and hypospadias and pubertal development in a large cohort study.Boys i
Seminal plasma is a rich source of proteins and serves as an ideal sample for proteomic analysis of male infertility.In varicoceleassociated infertility,the contributory role of seminal plasma proteins specific to unilateral and bilateral varicocele is no
The aim of this study was to validate the effectiveness of targeted microsurgical spermatic cord denervation (MSCD) of the trifecta nerve complex in comparison to traditional full MSCD with complete skeletonization of the spermatic cord in men with chroni