【摘 要】
：
目的 研究捐献者血常规参数对外周血造血干细胞采集效果的影响,及如何通过这些参数预测采集时机.方法 收集2018年1月至2020年8月本院使用血细胞分离机进行外周血造血干细胞采集的249例捐献者捐献前血常规参数及采集后产品的单个核细胞总数,以产品每升循环血量单个核细胞总数为采集效果的主要评价指标,研究其与采集前血常规参数间是否存在相关关系,并对相关影响因素分析采用多元线性回归分析.收集2020年9～10月本院进行外周血造血干细胞采集捐献者血常规参数代入方程,所得值为预测值,并与实际产品所得的实际值使用独立样
【机 构】
：
南方医科大学珠江医院输血科,广东广州,510282;南部战区总医院输血科
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目的 研究捐献者血常规参数对外周血造血干细胞采集效果的影响,及如何通过这些参数预测采集时机.方法 收集2018年1月至2020年8月本院使用血细胞分离机进行外周血造血干细胞采集的249例捐献者捐献前血常规参数及采集后产品的单个核细胞总数,以产品每升循环血量单个核细胞总数为采集效果的主要评价指标,研究其与采集前血常规参数间是否存在相关关系,并对相关影响因素分析采用多元线性回归分析.收集2020年9～10月本院进行外周血造血干细胞采集捐献者血常规参数代入方程,所得值为预测值,并与实际产品所得的实际值使用独立样本t检验进行验证.结果 通过相关分析得出血红蛋白、红细胞计数、红细胞比容、白细胞计数、中性粒细胞、淋巴细胞、单核细胞和血小板计数等参数与每升循环血量单个核细胞总数存在统计学上相关(P＜0.05).作图得出淋巴细胞、单核细胞、血小板计数和白细胞计数与每升循环血量单个核细胞总数存在明显线性关系.把每升循环血量单个核细胞总数设为(Y),以淋巴细胞(X1)、单核细胞(X2)、血小板计数(X3)、白细胞计数(X4)、和中性粒细胞等相关变量为因变量进行逐步多元线性回归,得出回归方程为:Y=9.814+3.131X1+1.666X2+0.020X3+0.124X4.验证结果,预测值与计算值间不存在统计学差异(P＞0.05).结论 使用捐献者的淋巴细胞、单核细胞、血小板计数和白细胞计数这四个采集前血常规数据,可有效预测采集效率,从而选择最合适的采集时机.
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