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目的:观察安宫牛黄丸对自发性高血压大鼠脑出血后Bcl-2 mRNA表达及行为学变化的影响。方法:自发性高血压大鼠随机分为空白对照组、模型组、西药治疗组、中药治疗组、中西药结合治疗组,以自体血制作大鼠脑出血模型于1、2、3d观察大鼠神经行为学评分,RT-PCR测定Bcl-2 mRNA的表达。结果:与空白对照组相比,模型组与各治疗组大鼠造模后行为学评分与Bcl-2 mRNA表达增加(P<0.05)。与其它3组相比,中西药结合治疗组大鼠造模后2d和3d大鼠行为学评分明显降低(P<0.05),Bcl-2 mRNA表达明显增加(P<0.05),中药组与西药治疗组行为学评分低于模型组(P<0.05),Bcl-2 mRNA表达明显高于模型组(P<0.05),中药组与西药治疗组间相比差异无显著性。结论:安宫牛黄丸能够有效改善神经功能缺损症状,提高Bcl-2 mRNA的表达。以早期与常规西药联合应用疗效更好。
Objective: To observe the effect of Angong Niuhuang Wan on the expression of Bcl-2 mRNA and the change of behavior after cerebral hemorrhage in spontaneously hypertensive rats. Methods: Spontaneous hypertensive rats were randomly divided into blank control group, model group, western medicine treatment group, traditional Chinese medicine treatment group, combination of Chinese and western medicine treatment group, autologous blood production rat model of intracerebral hemorrhage was observed at 1, Neurobehavioral score, Bcl-2 mRNA expression by RT-PCR. Results: Compared with the blank control group, the behavior scores and the expression of Bcl-2 mRNA in model group and each treatment group increased (P <0.05). Compared with the other three groups, the scores of behavioral scores of rats in model group decreased significantly (P <0.05) and the expressions of Bcl-2 mRNA increased significantly (P <0.05) Behavioral score in the treatment group was lower than that in the model group (P <0.05), and the expression of Bcl-2 mRNA in the treatment group was significantly higher than that in the model group (P <0.05). There was no significant difference between the TCM group and the western medicine group. Conclusion: Angong Niuhuang Wan can effectively improve the symptoms of neurological deficit and increase the expression of Bcl-2 mRNA. In early and conventional Western medicine combined effect is better.