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为了研究和探讨广藿香连作障碍成因及其作用机制,以广藿香扦插苗(2、8周龄苗)为试材,采用盆栽试验法,研究了广藿香重茬土壤、枯叶腐解液对其扦插苗叶片生理生化指标的影响。结果表明:2、8周苗龄广藿香扦插苗培育在广藿香重茬土壤和含不同浓度的枯叶腐解液的培养基质中60d,叶片过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性均较对照土呈升高的变化趋势,且随枯叶腐解液浓度的增加而升高,2、8周幼苗叶片CAT、POD、SOD活性均呈含高浓度枯叶腐解液的土壤>重茬土>含低浓度枯叶腐解液的土壤>对照土的变化规律;丙二醛(MDA)含量也较对照土显著升高,呈含高浓度枯叶腐解液的土壤>含低浓度枯叶腐解液的土壤>重茬土>对照土的变化规律。广藿香植株枯叶腐解物导致的化感自毒作用可能是其连作障碍的主要因素之一。
In order to study and discuss the cause and mechanism of continuous cropping obstacle in patchouli, Patchouli cuttings (2 and 8 weeks old seedlings) were used as materials to study the effects of patchouli dry soil and dead leaf decay Effect of Liquid on the Physiological and Biochemical Indexes of Cutting Seedling Leaves. The results showed that the seedlings of patchouli seedlings aged 2 and 8 weeks were cultivated for 60 days in the soil of patchouli and the medium containing different concentrations of decomposed leaf litter. The contents of catalase (CAT), peroxidase (POD) ) And superoxide dismutase (SOD) in leaves of seedlings increased as compared with the control soil, and increased with the increase of the concentration of litter decomposition. The activity of CAT, POD and SOD in the leaves of 2 and 8 weeks Soil with high concentration of foliar decomposing liquid> soil with repeated concentration of stubble> soil with lower concentration of decomposing loam> control soil; the content of malondialdehyde (MDA) was also significantly higher than that of the control soil, Soil with decomposed loam> Soil with low concentration of foliar decomposing liquid> Variety of soil under repeated stubble> control soil. Allelochemical and autotoxic effects caused by dead leaves and decomposers of patchouli plants may be one of the main factors of their continuous cropping obstacle.