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目的研究促红细胞生成素(EPO)及其受体(EPOR)在非小细胞肺癌(NSCLC)中的表达情况及其对NSCLC发生发展的影响。方法 qPCR、western blot检测EPO/EPOR在9种肺癌细胞系中的表达水平;MTT法检测rhEPO促细胞增殖的作用,流式细胞术检测rhEPO对细胞凋亡和周期的影响,Transwell检测细胞迁移;qPCR、western blot检测rhEPO对Cyclin D1和Cyclin A表达水平的影响。结果 EPO/EPOR在部分肺癌细胞中高表达,rhEPO促进高表达EPO/EPOR的肺癌细胞的增殖,而对肺癌细胞凋亡和迁移无影响,rhEPO诱导Cyclin D1表达,使用Jak2/Stat5抑制剂或干涉Jak2/Stat5后rhEPO作用消失。结论高表达EPO/EPOR非小细胞肺癌通过Jak2/Stat5/Cyclin D1通路促进自身增殖。
Objective To investigate the expression of erythropoietin (EPO) and its receptor (EPOR) in non-small cell lung cancer (NSCLC) and its effect on the development of NSCLC. Methods The expression of EPO / EPOR in nine lung cancer cell lines was detected by qPCR and western blot. The proliferation of rhEPO cells was detected by MTT assay. The effect of rhEPO on apoptosis and cell cycle were detected by flow cytometry. The effect of rhEPO on the expression of Cyclin D1 and Cyclin A by qPCR and western blot. Results EPO / EPOR was overexpressed in some lung cancer cells. RhEPO promoted the proliferation of lung cancer cells with high expression of EPO / EPOR, but had no effect on the apoptosis and migration of lung cancer cells. The expression of Cyclin D1 was induced by rhEPO, and Jak2 / Stat5 inhibitor or Jak2 / Stat5 rhEPO disappeared. Conclusion Highly expressed EPO / EPOR non-small cell lung cancer promotes self proliferation via the Jak2 / Stat5 / Cyclin D1 pathway.