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目的:探讨川芎不同提取物在等生药量下对异丙肾上腺素(ISO)致心肌缺血大鼠的治疗作用。方法:将正常成年大鼠分为对照组、模型组、消心痛组和川芎水提物、川芎醇提物、川芎挥发油、川芎总生物碱、川芎总酚酸组,给予预防性灌胃相应药液5d,其中各川芎提取物组给药量均相当于生药3.33g/kg,消心痛组给予消心痛9mg/kg。然后腹腔注射ISO建立大鼠急性心肌缺血模型,测定造模后1h各组大鼠心电图,试剂盒测定血清乳酸脱氢酶(LDH)、超氧化物歧化酶(SOD)、天冬氨酸转氨酶(AST)、肌酸激酶(CK)活性和丙二醛(MDA)、一氧化氮(NO)、肌钙蛋白(c Tn T)的含量,蛋白免疫印迹法(western blot)测定心肌组织Bax、Bcl-2蛋白表达水平。结果:模型组大鼠心电图出现显著的J点下移和T波倒置现象,血清LDH、AST、CK活性和MDA、c Tn T含量均显著升高,SOD、NO明显下降,心肌Bax表达升高而Bcl-2表达下降,Bax/Bcl-2比例显著下降;川芎挥发油能减轻模型大鼠J点下移现象,其余各组有减缓J点下移趋势的作用,而各组均能显著改善T波倒置现象;川芎提取物能显著降低模型大鼠血清LDH、AST、MDA、CK、c Tn T活性或含量,且川芎水提物、醇提物、挥发油能纠正心肌缺血时SOD、NO的减少;各提取物组均能降低心肌Bax的表达而升高Bcl-2表达水平,从而显著降低Bax/Bcl-2比例。结论:川芎对ISO所致心肌缺血有保护作用,以川芎水提物、川芎醇提物和川芎挥发油效果最佳。
Objective: To investigate the therapeutic effects of different extracts of Chuanxiong rhizome on isoproterenol-induced myocardial ischemia in rats at equal doses. Methods: The normal adult rats were divided into control group, model group, XiaoXiaoTeng group and Chuanxiong water extract, Chuanxiong alcohol extract, volatile oil of Rhizoma Chuanxiong, total rhizoma ligusticiformin, Ligusticum chuanxiong total phenolic acid group, given prophylactic gavage Liquid 5d, wherein the Chuanxiong extract group dose equivalent to crude drug 3.33g / kg, anti-Xiao pain group given to relieve pain 9mg / kg. The model of acute myocardial ischemia was established by intraperitoneal injection of ISO. The electrocardiogram of rats in each group was determined 1 h after the model was established. The serum LDH, SOD and aspartate aminotransferase (AST), creatine kinase (CK) activity, malondialdehyde (MDA), nitric oxide (NO) and cTn T were detected by Western blotting. The expressions of Bax, Bcl-2 protein expression level. Results: The electrocardiogram (ECG) of rats in model group showed significant J point down shift and T wave inversion. The activities of serum LDH, AST and CK, the content of MDA and cTn T were significantly increased, the levels of SOD and NO were significantly decreased and the expressions of Bax in myocardium were increased While the Bcl-2 expression decreased and the ratio of Bax / Bcl-2 decreased significantly. The volatile oil of Chuanxiong Hort could alleviate the J point down-shift in the model rats, while the other groups had the effect of slowing down the J point down trend, while each group could significantly improve T Wave inversion; Chuanxiong extract can significantly reduce the serum LDH, AST, MDA, CK, cTn T activity or content, and Chuanxiong water extract, alcohol extract, volatile oil can correct myocardial ischemia, SOD, NO Decreased; each extract group can reduce the expression of Bax and increase the expression of Bcl-2, thereby significantly reducing the Bax / Bcl-2 ratio. Conclusion: Chuanxiong has a protective effect on myocardial ischemia induced by ISO. Chuanxiong water extract, Chuanxiong alcohol extract and Ligusticum chuanxiong volatile oil have the best effect.