论文部分内容阅读
千粒重是油菜重要的产量相关性状之一,构建油菜遗传连锁图谱是研究其产量性状基因的前提。本研究利用小孢子培养技术,选育出了甘蓝型油菜大粒品系(G-42)和小粒品系(7-9)的纯合DH系DH-G-42和DH-7-9,其千粒重分别为6.24 g和2.42 g,二者比值达2.58。以DH-G-42为母本、DH-7-9为父本,构建了含190个单株的F2遗传作图群体,利用SSR和SRAP标记技术绘制遗传连锁图谱,该图谱共包含20个连锁群,涉及128个SSR标记和100个SRAP标记,图谱总长1546.6cM,标记间平均图距为6.78cM。本研究共检测到3个与千粒重性状相关的QTL,分别位于A9和C1连锁群,其中qSW-A9-1和qSW-A9-2贡献率分别达到10.98%和27.45%,均可视为控制粒重的主效QTL。本研究为后续进行油菜千粒重性状QTL的精细定位分析、分子标记辅助选择育种及新基因的克隆等奠定了基础。
Thousand grain weight is one of the important yield-related traits in rape. The construction of rape genetic linkage map is a prerequisite for the study of the yield trait gene. In this study, DH-G-42 and DH-7-9 homozygous DH strains of DH-G-42 and DH-7-9 were bred in microspore culture, 6.24 g and 2.42 g, the ratio between the two reached 2.58. Using DH-G-42 as the female parent and DH-7-9 as the male parent, F2 genetic mapping population containing 190 individuals was constructed and the genetic linkage map was drawn using SSR and SRAP markers. The map contains 20 The linkage group involved 128 SSR markers and 100 SRAP markers with a total length of 1546.6 cM and a mean distance between markers of 6.78 cM. Three QTLs associated with 1000-grain weight trait were detected in the A9 and C1 linkage groups, of which qSW-A9-1 and qSW-A9-2 contributed 10.98% and 27.45%, respectively. Heavy main effect QTL. This study laid the foundation for the subsequent fine mapping of QTL for QTLs, molecular marker-assisted breeding and cloning of new genes.