目的　初步探讨抑癌基因 p5 3、nm2 3、p1 6在儿童胚芽细胞肿瘤中的表达及意义。 方法　对 30例儿童胚芽细胞肿瘤石蜡标本进行免疫组织化学染色 ,观察肿瘤组织中抑癌基因 p5 3、nm2 3、p1 6的表达情况。 结果　在 30例肿瘤标本中有较高的 p5 3阳性表达率和p1 6阴性表达率 ,均达 6 3.3% (1 9/30 ) ,有约 5 0 %的病例呈nm2 3阳性表达 (1 6 /30 ) ,但三种抑癌基因的表达与发病年龄、临床分期、预后及肿瘤部位无明显相关性 (P >0 .0 5 )。结论　儿童胚芽细胞肿瘤的发生发展可能与抑癌基因p5 3的突变和 p1 6的失活存在一定的关联 Objective To investigate the expression and significance of tumor suppressor genes p5 3, nm23 and p16 in children with germ cell tumors. Methods Immunohistochemical staining of 30 cases of germ cell tumor paraffin specimens was performed to observe the expression of tumor suppressor genes p5 3, nm23 and p16 in tumor tissues. Results The high positive rate of p5 3 and the negative rate of p16 in all 30 tumor samples were 63.3% (19/30), and about 50% of them were positive for nm23 (16 / 30), but there was no significant correlation between the expression of the three tumor suppressor genes and the age of onset, clinical stage, prognosis and tumor location (P> 0.05). Conclusion The occurrence and development of children’s germ cell tumor may be related to the mutation of the tumor suppressor gene p5 3 and the inactivation of p1 6