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目的:研究白杨素对老年冠状动脉的保护作用及其机制。方法:利用微血管张力记录仪观察白杨素对老年大鼠冠状动脉肌张力的影响,利用Langedorff实验系统观察白杨素对大鼠离体心脏冠脉流量的影响。结果:1.白杨素(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)对冠状动脉血管环静息肌张力没有显著影响;2.白杨素或阳性对照药物芦丁(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)对高钾预收缩的青年大鼠冠脉血管环的最大舒张百分比为(30.3±4.2)%和(40.4±3.5)%,二者比较有统计学差异(P<0.05);白杨素或芦丁(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)对内皮素预收缩的青年大鼠冠脉血管环的最大舒张百分比为(31.7±3.3)%和(42.3±4.2)%,二者比较有统计学差异(P<0.05);白杨素或芦丁(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)对高钾预收缩的老年大鼠冠脉血管环的最大舒张百分比为(58.9±4.1)%和(65.5±4.2)%,二者比较有统计学差异(P<0.05);白杨素或芦丁(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)对内皮素预收缩的老年大鼠冠脉血管环的最大舒张百分比为(67.6±5.2)%和(78.2±3.2)%,二者比较有统计学差异(P<0.05);3.白杨素(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)浓度依赖性舒张高钾和内皮素预收缩的青年和老年大鼠的冠脉血管环,对高钾预收缩冠脉环的最大舒张百分比分别为(30.3±4.2)%(青年)和(58.9±4.1)%(老年),两组之间有显著性差异(P<0.05);对内皮素预收缩冠脉环的最大舒张百分比分别为(31.7±3.3)%(青年)和(67.6±5.2)%(老年),两组之间有显著性差异(P<0.05);4.提前孵育电压依赖性钾通道阻断剂4-AP(4-氨基吡啶,10~(-3)mol/L),使白杨素对高钾或内皮素预收缩老年大鼠冠脉的舒张幅度分别降低了(17.1±3.2)%和(19.4±4.2)%;而提前孵育钙激活的钾通道阻断剂TEA(四乙基铵,10~(-4)mol/L),对高钾或内皮素预收缩老年大鼠冠脉的舒张幅度没有产生显著影响;5.白杨素(10~(-6),3×10~(-6),10~(-5),3×10~(-5),10~(-4)mol/L)浓度依赖性增加青年和老年大鼠离体心脏的冠脉流量,最大增加幅度分别为(17.3±3.1)%(青年)和(25.4±4.2)%(老年)。结论:白杨素对老年大鼠冠状动脉有明显的舒张作用,其舒张作用与激活电压依赖性钾通道有关。
Objective: To study the protective effect of chrysin on coronary artery in aged patients and its mechanism. Methods: The effects of chrysin on the muscle tone of coronary artery in aged rats were observed by capillaris tension recorder. The effects of chrysin on coronary flow in isolated rat hearts were observed by Langedorff experimental system. Results: 1. The results of chlortin (10 -6, 3 × 10 -6, 10 -5, 3 × 10 -5, 10 -4 mol / L) There was no significant effect on the resting muscle tone of coronary artery rings; 2. Chrysin or positive control drug rutin (10 -6, 3 × 10 -6, 10 -5, (-5) and (10 -4 mol / L), respectively, were (30.3 ± 4.2)% and (40.4 ± 3.5)% in young rats with high K precontracted, respectively (P <0.05). There was a significant difference between chrysin and rutin (10 -6, 3 × 10 -6, 10 -5, 3 × 10 -5, (-4 mol / L), the maximum relaxation percentage of coronary rings in precontracted young rats was (31.7 ± 3.3)% and (42.3 ± 4.2)%, respectively, with statistical significance (P < 0.05), while those of chrysin or rutin (10 -6, 3 × 10 -6, 10 -5, 3 × 10 -5, 10 -4 mol / L) (58.9 ± 4.1)% and (65.5 ± 4.2)%, respectively, which were significantly different between the two groups (P <0.05). Chrysin or Lo Endothelin is pre-contracted by the combination of small doses of 10 ~ (-6), 3 × 10 ~ (-6), 10 ~ (-5), 3 × 10 ~ (-5), 10 ~ (-4) mol / L The maximum percentage of coronary vasodilatation in aged rats was (67.6 ± 5.2)% and (78.2 ± 3.2)%, respectively, with statistical significance (P <0.05 ); 3. The concentrations of chrysin (10 -6, 3 × 10 -6, 10 -5, 3 × 10 -5, 10 -4 mol / L) The maximum relaxation rate of pre-contracted coronary artery rings in high-potassium precontracted coronary artery rings was (30.3 ± 4.2)% (youth) and (58.9 ± 4.1) ) (%) (Elderly), with significant difference between the two groups (P <0.05). The maximum relaxation percentage of pre-contracted coronary artery rings of endothelin were (31.7 ± 3.3)% and 67.6 ± 5.2% (P <0.05); 4. Early incubation of voltage-dependent potassium channel blocker 4-AP (4-aminopyridine, 10-3 mol / L) (17.1 ± 3.2)% and (19.4 ± 4.2)%, respectively, of coronary artery in prematurely aged rats with high potassium or endothelin, whereas premature incubation of calcium-activated potassium channel blocker TEA (10 ~ (-4) mol / L) had no significant effect on the amplitude of coronary diastolic of precontracted aged rats with high potassium or endothelin.5. 10 ~ (-6), 10 ~ (-5), 3 × 10 ~ (-5) and 10 ~ (-4) mol / L increased the coronary flow in isolated heart of young and old rats in a concentration- The maximum increases were (17.3 ± 3.1)% (youth) and (25.4 ± 4. 2)% (old age). CONCLUSION: Chrysin has obvious relaxation effect on coronary artery in aged rats, and its relaxation is related to the activation of voltage-dependent potassium channel.