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目的:观察二联活菌洁阴制剂对家兔实验性念珠菌性阴道炎和淋病性阴道炎的疗效。方法:用致病菌直接注入新西兰大白兔阴道内制成相应的阴道炎模型。每种模型随机分5组:模型组(给予生理氯化钠溶液)、高、中、低三个剂量的二联活菌洁阴制剂治疗组(剂量分别为7×10~5,7×10~4,7×10~3CFU·mL~(-1))和阳性对照组(念珠菌阴道炎模型中选用14mg·kg~(-1)氟康唑做对照,淋病性阴道炎模型中选用140mg·kg~1)大观霉素做对照),每组6只,治疗7d。治疗d8取阴道拭子涂片,Gram’s染色镜检,检查阴道洁度;选择性培养基培养进行细菌学检测;分别处死大白兔,取阴道组织HE染色行病理学检查。结果:二联活菌中、高剂量组对家兔实验性念珠菌性阴道炎和淋病性阴道炎的治愈率均为83%,与氟康唑和大观霉素组相近(100%)。与模型组比较,二联活菌中、高剂量组和阳性对照组均可明显抑制念珠菌和淋球菌的生长;与阳性对照组比较,二联活菌中、高剂量组还显著增加阴道益生菌(厌氧菌)。结论:二联活菌制剂对家兔实验性念珠菌性和淋病性阴道炎有确切的疗效,而且可重建阴道内正常菌群微环境。
OBJECTIVE: To observe the curative effect of two live bacteria Jieyin preparations on experimental Candida vaginitis and gonococcal vaginitis in rabbits. Methods: The vagina of New Zealand white rabbits was directly injected with pathogens to make the corresponding model of vaginitis. Each model was randomly divided into 5 groups: model group (given physiological sodium chloride solution), high, medium and low doses of two viable Jieyang preparations treatment group (dose were 7 × 10 ~ 5, 7 × 10 ~ 4,7 × 10 ~ 3CFU · mL ~ (-1)) and positive control group (Candida vaginitis model selected 14mg · kg -1 fluconazole as control, gonococcal vaginitis model selected 140mg · Kg ~ 1) spectinomycin control), each group of 6, treatment 7d. Treated with vaginal swab smear d8 smear, Gram’s stained microscopy, check vaginal cleanliness; selective culture medium for bacteriological testing; were killed rabbits, take vaginal tissue HE staining pathological examination. Results: The cure rates of both experimental and pretreated vaginal and gonococcal vaginitis were 83% and 100% in fluconazole and spectinomycin groups respectively. Compared with the model group, the growth of Candida and Neisseria gonorrhoeae was significantly inhibited by the medium and high dose group and the positive control group. Compared with the positive control group, Bacteria (anaerobic bacteria). CONCLUSION: The viable oral preparation has definite curative effect on experimental candidiasis and gonococcal vaginitis in rabbits, and can reconstruct the normal microflora in the vagina.