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采用单菌核分离法得到大豆菌核病菌,利用菌丝生长速率法测定了7株大豆菌核病菌的生物学特性及对多菌灵和腐霉利的抗药性。结果表明:供试菌株菌丝生长的最适温度是15~20℃,而菌核的产生则在15℃左右最多;大豆菌核病菌丝生长最适的环境为中性和偏酸性;不同菌核及病残体埋入土壤不同深度后越冬处理,病残体上菌丝不能越冬,而菌核能够越冬,且埋入土层越深菌丝生长速度越慢,产生菌核的重量也降低;大豆菌核病菌对茄子、向日葵、油菜、大豆的致病力存在差异,H6菌株致病力最强;多菌灵对不同地理区域的菌株的EC50为0.5022~0.3525μg.mL-1,但均小于1μg.mL-1,其中最不敏感菌株EC50为最敏感菌株EC50的1.42倍,均为敏感菌株;腐霉利对不同地理区域的菌株的EC50为1.0230~0.4388μg.mL-1,但均小于10μg.mL-1,其中最不敏感菌株EC50为最敏感菌株EC50的2.33倍,均为敏感类型。尚未发现对多菌灵和腐霉利具有抗药性的菌株。
Single sclerotium separation method was used to obtain sclerotinia sclerotiorum, and the biological characteristics of seven Sclerotinia sclerotiorum and the resistance to carbendazim and procymidon were determined by mycelium growth rate method. The results showed that the optimal temperature for mycelial growth was 15 ~ 20 ℃, while the production of sclerotia was the highest at about 15 ℃. The optimum environment for mycelial growth of S. Sclerotiorum was neutral and acidic. Sclerotium and diseased bodies buried in different depths of the soil after winter treatment, the diseased mycelium can not wintering, while the sclerotium overwinter, and buried deeper soil mycelial growth slower, the weight of the sclerotium also decreased The pathogenicity of sclerotinia sclerotiorum to eggplant, sunflower, rapeseed and soybean was different, and the virulence of H6 strain was the strongest. The EC50 of carbendazim to different geographical regions was 0.5022 ~ 0.3525μg.mL-1 Were less than 1μg.mL-1, EC50 of the most sensitive strain was 1.42 times that of the most sensitive strain EC50, both of which were sensitive strains. The EC50 of pyrethrin against different geographical regions was 1.0230-0.4388μg.mL-1 Were less than 10μg.mL-1, the EC50 of the most sensitive strain EC50 2.33 times the most sensitive strains, are sensitive types. No strains that are resistant to carbendazim and tovermectin have been found.