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本文介绍了平喘固本方对致敏哮喘豚鼠肺组织β－肾上腺素能受体（β－AR）、腺苷酸环化酶(AC)活性及环磷酸腺苷(cAMP)含量的影响。豚鼠随机分成四组：对照组、平喘固本方组、酮替芬（西药对照）组和固本咳喘（中药对照）组，豚鼠肺中β－AR结合容量分别为22．68±6．19、29．77±5．84、24.64±2.64和26．13±6．52fmol／mg蛋白，平喘固本方组β－AR的结合位点（Rt值）大于对照组及酮替芬组，P＜0．05。肺组织中AC活性分别为9．66±2．36、13．1±2.4、8．4±2.4和9.02±2．81pmol·min－1／mg蛋白，平喘固本方组明显高于对照组、西药组和中药组，差异显著（P＜0．05）。cAMP含量分别为0．070±0．014、0096±0．027、0．098±0．029和0．097±0．015pmol／mg组织，用药各组均高于对照组，差异显著（P＜0．05）。提示平喘固本方可以提高哮喘豚鼠脑组织中β－AR功能，激活AC，使细胞中cAMP含量增加，使致敏哮喘豚鼠症状得以改善。
This article describes the effect of Pingchuan Guben recipe on β-AR, adenylyl cyclase (AC) activity and cyclic adenosine monophosphate (cAMP) content in lung tissue of sensitized asthmatic guinea pigs. Guinea pigs were randomly divided into four groups: control group, Pingchuan Gubenfang group, ketotifen (Western medicine control group) and Gubenkechuan (Chinese medicine control group). The lung bronchial β-AR binding capacity was 22.68±6 respectively. .19, 29.77±5.84, 24.64±2.64 and 26.13±6.52 fmol/mg protein, the binding site (Rt value) of β-AR in Pingchuan Gubenfang group was greater than that of the control group and ketotifen Group, P<0.05. The activities of AC in lung tissue were 9.66±2.36, 13.1±2.4, 8.4±2.4 and 9.02±2.81 pmol·min-1/mg protein, respectively, and those in Pingchuan Guben group were significantly higher than the control group. There was a significant difference between the group, western medicine group and traditional Chinese medicine group (P<0.05). The cAMP content was 0.070±0.014, 0096±0.027, 0.098±0.029, and 0.097±0.015 pmol/mg, respectively, and each group was higher than the control group. The difference was significant (P <0.05). It is suggested that Pingchuan Gubenfang can improve β-AR function in brain tissue of asthmatic guinea pigs, activate AC, increase cAMP content in cells, and improve the symptoms of sensitized asthmatic guinea pigs.